FastQCFastQC Report
Thu 26 May 2016
SRR1512709_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512709_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2068529
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT86070.41609278864352395No Hit
TATCAACGCAGAGTACTTTTTTTTT60230.29117309933774194No Hit
GGTATCAACGCAGAGTACTTTTTTT60130.2906896640076112No Hit
CTGTAGGACGTGGAATATGGCAAGA58110.2809242703389704No Hit
GTCCTACAGTGGACATTTCTAAATT57380.277395192429016No Hit
GTCCTAAAGTGTGTATTTCTCATTT52930.2558823202381983No Hit
CTTTAGGACGTGAAATATGGCGAGG50650.24485999471121747No Hit
GTCCTACAGTGTGCATTTCTCATTT35180.17007254913999273No Hit
ACGCAGAGTACTTTTTTTTTTTTTT28420.137392320823155No Hit
CTGAAGGACCTGGAATATGGCGAGA27780.1342983347103183No Hit
CTGTAGGACCTGGAATATGGCGAGA26010.12574152936700428No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21200.1024882899877159No Hit
TTTCTAAATTTTCCACCTTTTTCAG20880.10094129693129755No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC11800.013.6146344
ACCGCCC550.003070468812.0901558
GCGCCAC550.003077149212.08664713
GGCGAGG13000.011.75896419
TCCAACG1251.8475475E-811.39377818
AGGACCT21400.011.327885
GACGTGG15750.011.2786627
CCACCTT7450.011.0900513
ACAACGC600.005879502311.0826428
CCGATCA600.005879502311.0826429
CCTATAC1553.092282E-1011.0390463
CACCTTT7150.011.02248614
ATTTAGA8900.011.0091391
GGACCTG20950.010.9738596
GGTATCA18500.010.9010991
CCCCGTA1053.48916E-610.8514815
GTCCTAC31850.010.6925121
AATGTCC8800.010.6868338
AGGTTAA2500.010.63419219
GGACGTG32000.010.6282336
Produced by FastQC (version 0.11.5)