FastQCFastQC Report
Thu 26 May 2016
SRR1512708_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512708_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1841965
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT71440.3878466746110811No Hit
GGTATCAACGCAGAGTACTTTTTTT46250.2510905473231033No Hit
TATCAACGCAGAGTACTTTTTTTTT42410.230243245664277No Hit
GTACATGGGAAGCAGTGGTATCAAC32210.17486760063301965No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21280.11552879669266246No Hit
CCCATGTACTCTGCGTTGATACCAC21120.1146601591235447No Hit
GTACTTTTTTTTTTTTTTTTTTTTT20990.11395439109863652No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGTCG550.00308730612.08120716
TCTATAC1353.8016879E-1011.9458583
ACGGTAC1303.9176848E-710.2161423
GTTTAGG1354.0971645E-710.182781
CTACACT2550.010.0444424
TTAGGAC5500.010.0038593
TGGACCG1054.170336E-59.9381515
GTCCTAA4450.09.9294361
GTCTTAT1709.6988515E-99.819111
GTCTAAG1001.9763218E-49.8191091
GGCGAGG3750.09.60529719
GACGTGA4350.09.5954567
ATAGACC1106.944306E-59.4864173
CTTATAC1401.0792864E-69.4864173
TAGGACG8900.09.4864164
TGTCGAG1002.8253472E-49.47632618
GACGTGG4850.09.3886197
ACGTGAA5250.09.3658978
GTACTAA1053.227144E-49.3515331
TAGGAGA3050.09.3309014
Produced by FastQC (version 0.11.5)