FastQCFastQC Report
Thu 26 May 2016
SRR1512708_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512708_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1841965
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT91020.49414619713186736No Hit
GGTATCAACGCAGAGTACTTTTTTT64860.35212395458111306No Hit
TATCAACGCAGAGTACTTTTTTTTT60530.32861645036686365No Hit
GTACATGGGAAGCAGTGGTATCAAC35580.1931632794325625No Hit
ACGCAGAGTACTTTTTTTTTTTTTT31250.16965577521831304No Hit
GTACTTTTTTTTTTTTTTTTTTTTT25270.13719044607253666No Hit
CCCATGTACTCTGCGTTGATACCAC24840.1348559826055327No Hit
GAGTACTTTTTTTTTTTTTTTTTTT21020.11411726064284608No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCTAG657.912752E-411.7118961
ATACGGC657.9852855E-411.6991643
CGCCCTA1803.6379788E-1211.07779116
AGCGTAC1657.8216544E-1110.957721
GGTATCA25550.010.6146211
TCTAGAA3100.010.4265992
GACTCAT1553.6689016E-910.4181037
AGTGCGG750.002650607110.13211610
TTTATAC1701.542503E-910.0647223
TAGGACC3050.09.9730584
TCTACAC3150.09.9582173
TTAGGAC5300.09.8642713
GTATAAG1553.9590304E-89.822881
TACTAGA1106.7847395E-59.5066042
TTATACA2600.09.5055714
ATAAGAC1901.0404619E-99.505573
CGACTAA2201.6370905E-119.49499217
TATACAA3150.09.3549435
GTGCTAC1356.7026394E-69.1634731
CTAGACA2304.1836756E-119.0922854
Produced by FastQC (version 0.11.5)