FastQCFastQC Report
Thu 26 May 2016
SRR1512707_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512707_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2316124
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT61940.2674295504040371No Hit
GGTATCAACGCAGAGTACTTTTTTT39860.1720978669535828No Hit
TATCAACGCAGAGTACTTTTTTTTT36400.15715911583317646No Hit
GTCCTACAGTGGACATTTCTAAATT24440.10552112063084704No Hit
GTCCTAAAGTGTGTATTTCTCATTT23230.10029687529683212No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACCC604.1626347E-412.64148810
TATAGCG802.9066614E-511.8593755
CTTATAC1451.0004442E-1011.7775863
TCTATAC2000.011.3849993
AGGACGT13350.011.3707875
ATAGCGT600.00593402911.068996
GGACGTG13400.011.0453896
TCCAACG1553.274181E-1011.00507318
TATGTCG700.001502893210.847568516
GTATTAG2200.010.7057731
TAGGACC5450.010.6190374
GTGTAGG4400.010.4827371
GTCCTAA10000.010.4024431
ACTATCG1752.4374458E-1010.29512411
GGCGAGG6750.010.2525419
TAGGACG14850.010.2222224
AAGGCGT2151.8189894E-1210.1496396
GTCCTAG3100.010.1301941
GACGTGG8100.010.0733677
GTAGGAC14800.010.0003383
Produced by FastQC (version 0.11.5)