FastQCFastQC Report
Thu 26 May 2016
SRR1512707_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512707_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2316124
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT73230.31617478166108554No Hit
GGTATCAACGCAGAGTACTTTTTTT50820.21941830402862714No Hit
TATCAACGCAGAGTACTTTTTTTTT49090.21194892846842397No Hit
CTGTAGGACGTGGAATATGGCAAGA24840.10724814388176108No Hit
GTCCTACAGTGGACATTTCTAAATT24420.10543476946830135No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19800.011.2475921
TCGAAGT750.002655235610.13018913
TAGGACC5350.010.1273214
GACGTGA8350.09.8972697
CTACACT3950.09.8664354
ACCGTGC1554.071262E-89.8055268
TCCAACG1752.5993359E-99.76586618
GTCCTAA8150.09.6923561
TTAGGAC9850.09.6500133
GTCCTAC13650.09.5520541
GTCTTAG2900.09.5171941
GTATAAG2002.564775E-109.5171931
ACTCCGT900.00111380849.500136
ACTGTCC4700.09.4991048
ACCAGCG800.0045203789.4991037
ACGAAAT1502.6570706E-79.49807712
CGTGAAA9250.09.4477589
GATATAC4850.09.4190781
AGGACCT10000.09.3159635
AATCCCG1951.7007551E-99.25114119
Produced by FastQC (version 0.11.5)