FastQCFastQC Report
Thu 26 May 2016
SRR1512706_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512706_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2718503
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT64710.2380354187580444No Hit
GTATCAACGCAGAGTACTTTTTTTT62480.22983237465619863No Hit
CTGTAGGACGTGGAATATGGCAAGA61790.2272942130282733No Hit
GTCCTAAAGTGTGTATTTCTCATTT61630.22670565381020363No Hit
CTTTAGGACGTGAAATATGGCGAGG50750.18668362698146737No Hit
GGTATCAACGCAGAGTACTTTTTTT38640.14213705116382067No Hit
TATCAACGCAGAGTACTTTTTTTTT36220.13323509299051722No Hit
GTCCTACAGTGTGCATTTCTCATTT35990.13238903911454208No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCCG400.00532707814.2311225
TAGGACC10700.012.7681094
GTACTAG1551.4551915E-1112.0365211
TGTAGGA37750.011.38552952
GACGTGG20150.011.3474417
TTATACG600.005935840311.068654
GTAGGAC37600.010.9767413
GTCTAGG2600.010.9522591
CTGTAGG35750.010.8492591
GGCGAGG15400.010.76959219
AGGACGT38700.010.7376945
ACCTTTT9800.010.65249115
GGACGTG38050.010.6468486
CCAACGA1355.896618E-810.53026819
TCCAACG1909.094947E-1210.47252218
ACGTGGA21800.010.4117158
CCACCTT9900.010.35009913
GTCCTAC35450.010.3039971
CGTGGAA22150.010.2925519
GGTATCA20950.010.2645321
Produced by FastQC (version 0.11.5)