FastQCFastQC Report
Thu 26 May 2016
SRR1512706_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512706_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2718503
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT83070.30557258903153683No Hit
CTGTAGGACGTGGAATATGGCAAGA72210.2656241321050593No Hit
GTCCTACAGTGGACATTTCTAAATT68690.25267582930752697No Hit
GTCCTAAAGTGTGTATTTCTCATTT63820.23476155810753196No Hit
CTTTAGGACGTGAAATATGGCGAGG60800.22365250286646732No Hit
GGTATCAACGCAGAGTACTTTTTTT54640.20099297297078578No Hit
TATCAACGCAGAGTACTTTTTTTTT53420.19650520893300466No Hit
GTCCTACAGTGTGCATTTCTCATTT38050.13996673904718884No Hit
CTGAAGGACCTGGAATATGGCGAGA32620.11999251058395007No Hit
GTACATGGGAAGCAGTGGTATCAAC28090.10332892772235308No Hit
CTGTAGGACCTGGAATATGGCGAGA28010.10303464811331825No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC11700.012.3502184
ACCGTCC658.0334017E-411.6911648
GGCGAGG17400.011.45891319
TTAGGGC1251.816079E-811.4074913
ACGAAAT2150.011.04360212
GGACGTG40500.010.8604326
GGTATCA23350.010.8413611
GTATTAG1502.073648E-910.7856591
TAGAAAT10850.010.6892434
AGGACGT41950.010.6742615
AATGTCC10200.010.523488
TTAGGAC24850.010.4817323
TGTAGGA39650.010.4068532
GTCCTAC33850.010.4023661
GACGTGA22650.010.3168717
CTGTAGG39300.010.1705821
TTTAGGA26850.010.1627322
TGTGCGA750.002651621410.13196910
GCGATAA856.604496E-410.05746911
GGACCTG22600.010.0464026
Produced by FastQC (version 0.11.5)