FastQCFastQC Report
Thu 26 May 2016
SRR1512705_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512705_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2911049
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT84380.2898611462740751No Hit
GTCCTACAGTGGACATTTCTAAATT56200.19305755416689996No Hit
GGTATCAACGCAGAGTACTTTTTTT52630.1807939337331663No Hit
GTCCTAAAGTGTGTATTTCTCATTT51130.1756411520383202No Hit
TATCAACGCAGAGTACTTTTTTTTT49450.16987003654009258No Hit
CTGTAGGACGTGGAATATGGCAAGA48820.16770586822825723No Hit
CTTTAGGACGTGAAATATGGCGAGG43080.14798789027597956No Hit
GTACATGGGAAGCAGTGGTATCAAC36190.1243194463576532No Hit
GTCCTACAGTGTGCATTTCTCATTT33470.11497573555099896No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCCG551.9845241E-413.7988275
TAGGACC8900.013.43064
GTACTAG1653.6379788E-1211.898341
GTATTAT2550.011.5483891
GGCGAGG14300.011.20225419
TTAGGAC20400.010.6024083
GACGTGA15850.010.4742677
TGTAGGA31000.010.4656022
ACGGTCC1002.520809E-510.4037188
GTCTTAC2550.010.3935511
GTCCTAC29700.010.3779971
ACTGTTC6150.010.3037588
GTAGGAC30550.010.2781893
TAGGACA10250.010.2733954
GTATTAA3450.010.242921
AGGACCT19650.010.2350075
AGGACGT31800.010.202675
GGACGTG30950.010.1763566
TCCTACA32400.010.1305022
TAGAAAT8300.010.0581814
Produced by FastQC (version 0.11.5)