FastQCFastQC Report
Thu 26 May 2016
SRR1512705_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512705_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2911049
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT104290.35825573530366545No Hit
TATCAACGCAGAGTACTTTTTTTTT72800.25008167158986333No Hit
GGTATCAACGCAGAGTACTTTTTTT71770.2465434281594023No Hit
CTGTAGGACGTGGAATATGGCAAGA58140.19972181849223425No Hit
GTCCTACAGTGGACATTTCTAAATT56570.19432857365162867No Hit
GTCCTAAAGTGTGTATTTCTCATTT53940.1852940297466652No Hit
CTTTAGGACGTGAAATATGGCGAGG49320.16942346212653925No Hit
GTACATGGGAAGCAGTGGTATCAAC40980.14077399590319503No Hit
GTCCTACAGTGTGCATTTCTCATTT36080.12394157570003116No Hit
ACGCAGAGTACTTTTTTTTTTTTTT33800.11610934752386512No Hit
CTGTAGGACCTGGAATATGGCGAGA29440.10113192873084581No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC11300.012.0297524
GGCGAGG15150.011.03002819
AAATGTC8800.010.9020777
GTGTAGG4200.010.6514361
ATTTAGA9800.010.5866861
GAAATGT9350.010.5666446
TGTAGGA35200.010.5074092
CCAACGA1002.41546E-510.44405819
GTCCTAA17050.010.3836051
GGTATCA28700.010.38058951
TAGAAAT10350.010.28671554
CGTGCGC1303.853129E-710.22916910
TGGCGAG35500.010.13648618
TCCAACG1606.2373147E-910.08801118
CTGTAGG34900.09.9819471
TTTAGAA10400.09.9651052
GTAGGAC35800.09.9307083
AGGACGT36450.09.8582735
GACGTGG17350.09.8547057
AATGTCC10350.09.8198888