FastQCFastQC Report
Thu 26 May 2016
SRR1512704_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512704_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2502304
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT68120.2722291136488612No Hit
GTCCTACAGTGGACATTTCTAAATT43800.17503868434850442No Hit
GGTATCAACGCAGAGTACTTTTTTT42700.17064273565482052No Hit
GTCCTAAAGTGTGTATTTCTCATTT41970.1677254242490121No Hit
CTGTAGGACGTGGAATATGGCAAGA40160.16049209048940496No Hit
TATCAACGCAGAGTACTTTTTTTTT39990.1598127166003811No Hit
CTTTAGGACGTGAAATATGGCGAGG34920.13955138943949258No Hit
GTCCTACAGTGTGCATTTCTCATTT26540.10606225302760976No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC9650.013.5673264
CGCTAGC500.001520935813.27554310
CGCGCTC802.0605858E-613.02827418
GGTATCA17400.011.7902491
CTATACT3000.011.0685134
GTCCTAC23200.010.8735431
GTCCTAA14900.010.8698521
GGCGAGG11450.010.84378119
TGGCGAA1408.707502E-910.82869418
AGGACCT18050.010.7224865
GGCGAAA803.854214E-410.66270719
TGTAGGA28950.010.6506792
GTGTAGG4950.010.5098521
GTAGGAC28450.010.5043883
TCTATAC2900.010.468743
TCCTACA27100.010.3625092
AGGACGT27200.010.2546515
TGGCGAG26100.010.23747318
AATGCCG1502.5482223E-810.10678218
GTATAAC1853.583409E-1010.0810981
Produced by FastQC (version 0.11.5)