FastQCFastQC Report
Thu 26 May 2016
SRR1512704_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512704_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2502304
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT89100.35607184418839594No Hit
GGTATCAACGCAGAGTACTTTTTTT60130.24029854086473906No Hit
TATCAACGCAGAGTACTTTTTTTTT59580.23810056651789713No Hit
CTGTAGGACGTGGAATATGGCAAGA46790.18698767216133613No Hit
GTCCTACAGTGGACATTTCTAAATT44730.17875525915316443No Hit
GTCCTAAAGTGTGTATTTCTCATTT44600.1782357379439109No Hit
CTTTAGGACGTGAAATATGGCGAGG40400.1614512065680269No Hit
GTCCTACAGTGTGCATTTCTCATTT28640.11445451871555175No Hit
ACGCAGAGTACTTTTTTTTTTTTTT28080.11221658119876722No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC9900.012.9631124
CGTGCGC1302.6284397E-911.68951510
AAGGCGT2300.011.5645366
GCGTGCG1751.8189894E-1211.3972789
AGGACCT19200.011.1403975
GGTATCA20850.010.8219391
GGCGAGG12000.010.68153119
GGCGTGC2150.010.602338
TACACCG909.453975E-510.5627475
GGACCTG19150.010.5163526
TGTAGGA29900.010.4310082
GTGCGCC1553.6798156E-910.41686711
GTCCTAA14300.010.3860741
GTAGGAC29400.010.314643
TTAGGAC17150.010.1991613
AGGCGTG2800.010.1765477
CACCTTT6850.010.11944514
TGCGCCA1606.21003E-910.09053212
AGGACGT27150.010.0842135
CTGTAGG30100.010.0582741
Produced by FastQC (version 0.11.5)