FastQCFastQC Report
Thu 26 May 2016
SRR1512703_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512703_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2278329
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT68370.3000883542280329No Hit
GTCCTACAGTGGACATTTCTAAATT65230.2863063236257801No Hit
GTCCTAAAGTGTGTATTTCTCATTT63230.27752796018485476No Hit
CTGTAGGACGTGGAATATGGCAAGA54850.2407466173673776No Hit
CTTTAGGACGTGAAATATGGCGAGG50150.22011746328120305No Hit
GGTATCAACGCAGAGTACTTTTTTT44720.19628420653909068No Hit
TATCAACGCAGAGTACTTTTTTTTT41030.18008812599058346No Hit
GTCCTACAGTGTGCATTTCTCATTT39100.1716170052700905No Hit
CTGAAGGACCTGGAATATGGCGAGA26750.11741061102237649No Hit
CTGTAGGACCTGGAATATGGCGAGA26480.11622553195785157No Hit
GTCCTTCAGTGTGCATTTCTCATTT24270.10652544035562907No Hit
ATTTAGAAATGTCCACTGTAGGACG23180.10174123228032475No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC12750.013.616874
TGGCGAA1251.4697434E-912.12884418
CCAACGT907.66206E-611.58391519
CACCTTT9600.011.56554414
GTTTAGG3150.011.5323851
GTTTAGA3250.011.4796371
AGGACCT23600.011.2961575
CCACCTT9650.011.20713913
ATTTAGA9600.011.1476471
GGCGAGG19000.010.974236519
TTTAGAA12050.010.8652082
ACCTTTT10400.010.8583815
TAGAAAT11100.010.7692144
GTGTAGG4200.010.7531691
GGACCTG24650.010.7380076
GTGTAGA3750.010.73446751
TTAGAAA11600.010.7141893
GTCCTAA20750.010.6934611
TCCAACG1701.4551915E-1010.59044318
TGTAGGA39000.010.5577432
Produced by FastQC (version 0.11.5)