FastQCFastQC Report
Thu 26 May 2016
SRR1512703_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512703_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2278329
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT82610.36259030192742137No Hit
GTCCTAAAGTGTGTATTTCTCATTT64880.2847701100236182No Hit
GTCCTACAGTGGACATTTCTAAATT64440.2828388700666146No Hit
CTGTAGGACGTGGAATATGGCAAGA63470.2785813637977658No Hit
CTTTAGGACGTGAAATATGGCGAGG61080.26809121948586No Hit
GGTATCAACGCAGAGTACTTTTTTT57860.25395805434597024No Hit
TATCAACGCAGAGTACTTTTTTTTT56440.24772541630291323No Hit
GTCCTACAGTGTGCATTTCTCATTT42030.18447730771104615No Hit
CTGAAGGACCTGGAATATGGCGAGA33490.1469936958182949No Hit
CTGTAGGACCTGGAATATGGCGAGA31330.13751306330209553No Hit
ATTTAGAAATGTCCACTGTAGGACG27590.12109752366756513No Hit
TTTCTAAATTTTCCACCTTTTTCAG27520.12079028094713275No Hit
ACGCAGAGTACTTTTTTTTTTTTTT27100.11894682462453843No Hit
GTCCTTCAGTGTGCATTTCTCATTT25480.11183635023738889No Hit
GAATATGGCAAGAAAACTGAAAATC24010.10538425310830876No Hit
GTACATGGGAAGCAGTGGTATCAAC23370.10257517680721266No Hit
GTACTTTTTTTTTTTTTTTTTTTTT22900.10051226139859519No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCGC250.006049491518.9909817
TATTCCG707.2227613E-613.579295
TAGGACC11800.013.2915944
ATAGGAC3050.012.4662353
AGGACCG1501.4551915E-1112.0403045
AAGGCGT1302.6193447E-911.6916326
ACAACGC752.0782789E-411.3978398
GGACCGT1104.9751725E-711.22662456
GGTATCA20050.011.0124371
AGGACCT23750.011.0063725
GGCGAGG16950.010.98003519
TTATACT3150.010.8634334
TCGCGCG700.001494224910.8550849
CTACACT3650.010.6774154
AGGACGG1701.382432E-1010.6237985
GGACCTG23650.010.5638716
ACCGTGC909.5369236E-510.55355558
ATTATAC2350.010.5167273
CTGTAGG38350.010.3485731
ACCTTTT9700.010.27862415
Produced by FastQC (version 0.11.5)