##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512703_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2278329 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.822506319324383 33.0 31.0 34.0 30.0 34.0 2 31.951694421657276 34.0 31.0 34.0 30.0 34.0 3 32.05202453201447 34.0 31.0 34.0 30.0 34.0 4 35.49173714595214 37.0 35.0 37.0 33.0 37.0 5 35.288500036649666 37.0 35.0 37.0 33.0 37.0 6 35.144234656188814 37.0 35.0 37.0 32.0 37.0 7 35.14676984755055 37.0 35.0 37.0 32.0 37.0 8 35.14810591446626 37.0 35.0 37.0 32.0 37.0 9 36.771328899381956 39.0 37.0 39.0 33.0 39.0 10 36.57012705364326 39.0 37.0 39.0 32.0 39.0 11 36.68190195533656 39.0 37.0 39.0 32.0 39.0 12 36.634120006373095 39.0 37.0 39.0 32.0 39.0 13 36.63326148242857 39.0 37.0 39.0 33.0 39.0 14 37.91392200160732 40.0 38.0 41.0 33.0 41.0 15 37.908827478384374 40.0 38.0 41.0 33.0 41.0 16 37.88083064386223 40.0 38.0 41.0 33.0 41.0 17 37.859448745111 40.0 38.0 41.0 33.0 41.0 18 37.79527364133977 40.0 38.0 41.0 32.0 41.0 19 37.82028495445566 40.0 38.0 41.0 32.0 41.0 20 37.77917631737998 40.0 38.0 41.0 32.0 41.0 21 37.73536701679169 40.0 38.0 41.0 32.0 41.0 22 37.64592295493759 40.0 38.0 41.0 32.0 41.0 23 37.59367808600075 40.0 38.0 41.0 32.0 41.0 24 37.564831944815694 40.0 38.0 41.0 32.0 41.0 25 37.49995369413285 40.0 38.0 41.0 32.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5680219585434081 1101 2 0.501305203915777 1101 3 0.4830213177346607 1101 4 0.43604618545978013 1101 5 0.4940380543226013 1101 6 0.7079887680799786 1101 7 0.6378060141240951 1101 8 0.7428024678850065 1101 9 0.8482139289759374 1101 10 0.9661336231110482 1101 11 0.8981961705806469 1101 12 0.8191808259809505 1101 13 0.8016234123343509 1101 14 0.8761750918408495 1101 15 0.9160110717886809 1101 16 1.0064227494207643 1101 17 0.9380998375197152 1101 18 1.0091254691809297 1101 19 1.0260770689938 1101 20 1.0332466775437297 1101 21 1.1271672540154327 1101 22 1.1861788316849626 1101 23 1.0526908460665396 1101 24 1.001497355253349 1101 25 0.9543404105937654 1102 1 0.3796168763057288 1102 2 0.385339442685698 1102 3 0.38192254747288956 1102 4 0.25914757508099484 1102 5 0.24522612092916773 1102 6 0.42895810116385036 1102 7 0.43068952066398936 1102 8 0.372916171897387 1102 9 0.3215542926246684 1102 10 0.650659842218765 1102 11 0.5188112948301296 1102 12 0.47708865514428567 1102 13 0.4810193438227941 1102 14 0.6945641857805995 1102 15 0.6761572626617465 1102 16 0.6727380420576452 1102 17 0.615160456981215 1102 18 0.6873558707593475 1102 19 0.6659079162035013 1102 20 0.7212273895332473 1102 21 0.6846132935099263 1102 22 0.695175651034198 1102 23 0.5965655420337441 1102 24 0.7472196351166005 1102 25 0.6733073794505984 1103 1 0.3555166897062598 1103 2 0.4517996315936088 1103 3 0.3841290380563507 1103 4 0.28180498231028395 1103 5 0.4613948684043052 1103 6 0.568892310424566 1103 7 0.4742056701744062 1103 8 0.49306506672839134 1103 9 0.5230567068754866 1103 10 0.5890762475820566 1103 11 0.4896998545515814 1103 12 0.5255948975696967 1103 13 0.5727801117661571 1103 14 0.6277211794131361 1103 15 0.5785445815929364 1103 16 0.5758082765573889 1103 17 0.6258908880836884 1103 18 0.7599372916084732 1103 19 0.6502989671239945 1103 20 0.6350819188986208 1103 21 0.6105948644081067 1103 22 0.5611472186620787 1103 23 0.4935664846496053 1103 24 0.5016431966655617 1103 25 0.3948855877109949 1104 1 0.2702521204750674 1104 2 0.2705108167781063 1104 3 0.20951241437500556 1104 4 0.1660006601903774 1104 5 0.15102531877254677 1104 6 0.3488664261218162 1104 7 0.34665750954275154 1104 8 0.2179177217316024 1104 9 0.3717257136817551 1104 10 0.5548819663370068 1104 11 0.3865108712087846 1104 12 0.3748700439835986 1104 13 0.34775520510793 1104 14 0.3428523234434522 1104 15 0.2583538943553876 1104 16 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-1.808923943038458 2314 18 -1.7645129256313936 2314 19 -1.675367887717627 2314 20 -1.757751288373214 2314 21 -1.706899431374623 2314 22 -1.538620366916902 2314 23 -1.5063331903161554 2314 24 -1.7479840439399723 2314 25 -1.6121094888143688 2315 1 -0.48611164309114585 2315 2 -0.5040150929440088 2315 3 -0.5164262685118608 2315 4 -0.4698910102523044 2315 5 -0.4438757956477204 2315 6 -0.37867795260047643 2315 7 -0.5568181898476894 2315 8 -0.6431563161565705 2315 9 -0.7255531940090378 2315 10 -0.6674492293767997 2315 11 -0.7301105781476949 2315 12 -0.7414570793250164 2315 13 -0.7112281253645776 2315 14 -0.9776742745037481 2315 15 -0.9951532469967717 2315 16 -0.8971241519800088 2315 17 -0.9562051817853074 2315 18 -0.9421324874184975 2315 19 -0.86904662749388 2315 20 -0.8607616955756683 2315 21 -0.7878451789767809 2315 22 -0.6682870284085212 2315 23 -0.8181542659556058 2315 24 -0.7781120219332891 2315 25 -0.8131606095302999 2316 1 -1.1617798331444327 2316 2 -1.184078292125875 2316 3 -1.1118324470601664 2316 4 -0.9349224536817431 2316 5 -1.0767659801356615 2316 6 -4.655254702727344 2316 7 -2.2471894219518944 2316 8 -1.6018297379947413 2316 9 -1.6196805723173853 2316 10 -1.4178523134289165 2316 11 -1.4085219634950477 2316 12 -1.582076908037095 2316 13 -1.5443963023002993 2316 14 -1.725397502364359 2316 15 -1.8007566712560745 2316 16 -1.8964797671850917 2316 17 -1.8730355681638287 2316 18 -2.086092398139101 2316 19 -2.0932224508459925 2316 20 -2.243444060345439 2316 21 -2.3326382924797002 2316 22 -2.292009783537054 2316 23 -2.452229278941992 2316 24 -2.268204636943736 2316 25 -2.3276642645838024 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 18294.0 3 1.0 4 368.0 5 3075.0 6 4016.0 7 3302.0 8 2541.0 9 2223.0 10 2183.0 11 2054.0 12 1980.0 13 1946.0 14 1956.0 15 1967.0 16 2203.0 17 2133.0 18 2464.0 19 2641.0 20 3442.0 21 4407.0 22 5768.0 23 7145.0 24 8861.0 25 10595.0 26 12034.0 27 13824.0 28 16225.0 29 19877.0 30 25354.0 31 33652.0 32 43529.0 33 58395.0 34 77733.0 35 107342.0 36 160913.0 37 288811.0 38 1325075.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.21444145522322 19.446422605545884 12.919282235234125 25.419853703996765 2 14.70160352874657 20.81365211924972 41.50188588466253 22.98285846734118 3 18.54003526268594 25.553772084716474 30.36892389115005 25.537268761447535 4 12.376259135853896 17.594564948783034 38.52041633728359 31.50875957807948 5 12.515393811369298 38.49698406512609 36.18893385417101 12.798688269333594 6 31.08017795857001 37.74005613458248 18.295245773220504 12.884520133627003 7 27.29969622905181 31.7424176901027 23.062313216429008 17.895572864416476 8 24.389198758025767 38.11857236185847 20.82229863579527 16.669930244320497 9 26.59227810754803 14.534701977450739 20.562458290892486 38.31056162410874 10 15.290688918929717 28.85428388407128 34.97526607573362 20.879761121265382 11 33.74495227465422 21.730432915581964 25.971949202440737 18.552665607323082 12 23.763559550498652 24.421328921567337 32.59679497142977 19.218316556504238 13 29.53591591960734 22.11746318848724 25.91574400084291 22.430876891062507 14 20.281802171328835 23.025198722417223 27.91604405869576 28.77695504755818 15 22.296655800105608 32.64076746861507 23.267886700409043 21.79469003087028 16 21.10174818237842 27.84068663168078 29.501126302094764 21.556438883846035 17 21.0169745953868 29.21545880742856 28.54714967868403 21.22041691850061 18 20.961500646103715 26.856714211472553 31.714255365469967 20.46752977695376 19 23.560807833236478 26.383360868580496 28.15951598829298 21.896315309890042 20 23.712773703885613 27.26678192657865 28.774948657546823 20.245495711988916 21 22.335931289993674 26.50819964983108 27.754376123904844 23.401492936270397 22 23.227709674148677 28.663100571520367 27.610302202203474 20.498887552127478 23 21.33989428216908 28.464106808103658 28.797728510676023 21.398270399051235 24 23.125950258259564 26.751372058808037 29.37285588378846 20.749821799143934 25 22.08135875020684 27.663915088646107 29.01310565769913 21.241620503447923 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 442.0 1 442.0 2 669.5 3 897.0 4 897.0 5 897.0 6 2162.5 7 3428.0 8 3428.0 9 3428.0 10 4512.0 11 5596.0 12 5596.0 13 5596.0 14 8278.5 15 10961.0 16 10961.0 17 10961.0 18 19576.5 19 28192.0 20 28192.0 21 28192.0 22 45488.5 23 62785.0 24 62785.0 25 62785.0 26 92461.5 27 122138.0 28 122138.0 29 122138.0 30 157876.0 31 193614.0 32 193614.0 33 193614.0 34 213919.5 35 234225.0 36 234225.0 37 234225.0 38 246083.5 39 257942.0 40 257942.0 41 257942.0 42 267818.0 43 277694.0 44 277694.0 45 277694.0 46 287585.5 47 297477.0 48 297477.0 49 297477.0 50 281880.0 51 266283.0 52 266283.0 53 266283.0 54 232495.5 55 198708.0 56 198708.0 57 198708.0 58 169923.0 59 141138.0 60 141138.0 61 141138.0 62 115862.5 63 90587.0 64 90587.0 65 90587.0 66 69870.5 67 49154.0 68 49154.0 69 49154.0 70 36213.5 71 23273.0 72 23273.0 73 23273.0 74 16197.0 75 9121.0 76 9121.0 77 9121.0 78 6240.0 79 3359.0 80 3359.0 81 3359.0 82 2165.5 83 972.0 84 972.0 85 972.0 86 622.0 87 272.0 88 272.0 89 272.0 90 168.0 91 64.0 92 64.0 93 64.0 94 34.0 95 4.0 96 4.0 97 4.0 98 3.5 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15313855022694264 2 0.015493811473233234 3 0.0 4 0.0035113453763701377 5 0.09884437234481938 6 0.04147776725837226 7 0.027476277570096328 8 0.02918805844107677 9 0.028485789365802743 10 0.030197570236783187 11 0.03590350647338466 12 0.03436729287122273 13 0.022340934957155 14 0.012158033365681603 15 0.0037746962795978983 16 0.0033796699247562578 17 0.0018434563225943224 18 0.0025018335806637234 19 0.001623997236571189 20 0.0 21 0.0 22 8.778363440925344E-4 23 0.0 24 4.389181720462672E-5 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2278329.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.95199714729291 #Duplication Level Percentage of deduplicated Percentage of total 1 81.14937891974601 52.70814228099935 2 12.694937430184291 16.491230795007844 3 3.2176135157798926 6.269712716940802 4 1.1592042827531124 3.0117053306603956 5 0.5408733085700725 1.7565400797645114 6 0.3073219373979912 1.1976704160704914 7 0.19717072784370518 0.8964642786703803 8 0.12747016565042532 0.6623553468553104 9 0.09578241858343992 0.5599133440733164 >10 0.4457805878553466 5.100759145480187 >50 0.03181404289318413 1.4268593042989655 >100 0.02596420150168991 3.4593910960154255 >500 0.0036482515859059877 1.5619694186739626 >1k 0.0025672881530437357 2.9191867848806856 >5k 4.729215018764776E-4 1.9780996616086157 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 8261 0.36259030192742137 No Hit GTCCTAAAGTGTGTATTTCTCATTT 6488 0.2847701100236182 No Hit GTCCTACAGTGGACATTTCTAAATT 6444 0.2828388700666146 No Hit CTGTAGGACGTGGAATATGGCAAGA 6347 0.2785813637977658 No Hit CTTTAGGACGTGAAATATGGCGAGG 6108 0.26809121948586 No Hit GGTATCAACGCAGAGTACTTTTTTT 5786 0.25395805434597024 No Hit TATCAACGCAGAGTACTTTTTTTTT 5644 0.24772541630291323 No Hit GTCCTACAGTGTGCATTTCTCATTT 4203 0.18447730771104615 No Hit CTGAAGGACCTGGAATATGGCGAGA 3349 0.1469936958182949 No Hit CTGTAGGACCTGGAATATGGCGAGA 3133 0.13751306330209553 No Hit ATTTAGAAATGTCCACTGTAGGACG 2759 0.12109752366756513 No Hit TTTCTAAATTTTCCACCTTTTTCAG 2752 0.12079028094713275 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2710 0.11894682462453843 No Hit GTCCTTCAGTGTGCATTTCTCATTT 2548 0.11183635023738889 No Hit GAATATGGCAAGAAAACTGAAAATC 2401 0.10538425310830876 No Hit GTACATGGGAAGCAGTGGTATCAAC 2337 0.10257517680721266 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2290 0.10051226139859519 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 8.778363440925345E-5 0.0 0.0 0.0 6 0.0 8.778363440925345E-5 0.0 0.0 0.0 7 0.0 8.778363440925345E-5 0.0 0.0 0.0 8 0.0 8.778363440925345E-5 0.0 0.0 0.0 9 0.0 8.778363440925345E-5 0.0 0.0 0.0 10 0.0 8.778363440925345E-5 0.0 0.0 0.0 11 0.0 8.778363440925345E-5 0.0 0.0 0.0 12 0.0 8.778363440925345E-5 0.0 0.0 0.0 13 0.0 8.778363440925345E-5 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTCGC 25 0.0060494915 18.99098 17 TATTCCG 70 7.2227613E-6 13.57929 5 TAGGACC 1180 0.0 13.291594 4 ATAGGAC 305 0.0 12.466235 3 AGGACCG 150 1.4551915E-11 12.040304 5 AAGGCGT 130 2.6193447E-9 11.691632 6 ACAACGC 75 2.0782789E-4 11.397839 8 GGACCGT 110 4.9751725E-7 11.2266245 6 GGTATCA 2005 0.0 11.012437 1 AGGACCT 2375 0.0 11.006372 5 GGCGAGG 1695 0.0 10.980035 19 TTATACT 315 0.0 10.863433 4 TCGCGCG 70 0.0014942249 10.855084 9 CTACACT 365 0.0 10.677415 4 AGGACGG 170 1.382432E-10 10.623798 5 GGACCTG 2365 0.0 10.563871 6 ACCGTGC 90 9.5369236E-5 10.5535555 8 ATTATAC 235 0.0 10.516727 3 CTGTAGG 3835 0.0 10.348573 1 ACCTTTT 970 0.0 10.278624 15 >>END_MODULE