FastQCFastQC Report
Thu 26 May 2016
SRR1512702_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512702_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2984685
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT72130.2416670435908647No Hit
GTCCTACAGTGGACATTTCTAAATT69850.2340280465107708No Hit
CTGTAGGACGTGGAATATGGCAAGA60380.2022994051298546No Hit
GTCCTAAAGTGTGTATTTCTCATTT59520.1994180290382402No Hit
CTTTAGGACGTGAAATATGGCGAGG48290.16179261798146202No Hit
GGTATCAACGCAGAGTACTTTTTTT44320.14849138183761434No Hit
GTCCTACAGTGTGCATTTCTCATTT42030.1408188803843622No Hit
TATCAACGCAGAGTACTTTTTTTTT41240.13817203490485594No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC12000.013.0449544
TTACACG701.1053255E-412.197884
GTCCTAC36700.011.0234691
TAGAAAT10200.010.789414
GTCCTAT4100.010.7774431
GACCGGG803.8199985E-410.6731447
CGTGCGG803.839079E-410.6673910
AGGACCT24950.010.5329275
GGTATCA22950.010.5254221
TAGGACT4700.010.496524
GTAGGAC38350.010.4396743
GGACGTG36900.010.4385346
GACGTGG19150.010.4037617
AGGACGT37850.010.4021255
GGCGAGG15550.010.36159619
TCCTACA40750.010.3370172
TATGTCG1856.184564E-1110.2602816
GTACTAG1354.0990562E-710.1831321
TTTAGAA11000.010.177222
TGTAGGA41050.010.1459152
Produced by FastQC (version 0.11.5)