FastQCFastQC Report
Thu 26 May 2016
SRR1512702_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512702_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2984685
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT94100.31527615141966403No Hit
CTGTAGGACGTGGAATATGGCAAGA72660.24344277536825493No Hit
GTCCTACAGTGGACATTTCTAAATT72180.24183456545665621No Hit
GGTATCAACGCAGAGTACTTTTTTT61700.20672198238675102No Hit
GTCCTAAAGTGTGTATTTCTCATTT61230.2051472768483106No Hit
TATCAACGCAGAGTACTTTTTTTTT61190.2050132593556774No Hit
CTTTAGGACGTGAAATATGGCGAGG56700.1899697958075978No Hit
GTCCTACAGTGTGCATTTCTCATTT44100.14775428562813162No Hit
CTGTAGGACCTGGAATATGGCGAGA32250.10805160343553843No Hit
CTGAAGGACCTGGAATATGGCGAGA31960.10707997661394753No Hit
ACGCAGAGTACTTTTTTTTTTTTTT30060.1007141457138693No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC13850.012.9033894
TCCAACG1501.8189894E-1212.65995418
GCCGGTT1157.0964234E-811.56354411
GGCGAGG17150.011.40503119
TGTAGGA40350.011.003412
ATAGGAC4350.010.9262013
ACCGTGC1408.380084E-910.8555738
AGGACCT24500.010.6699495
CGACTAA1002.414483E-510.44446317
GTAGGAC41000.010.3868193
GGACCGT1106.0532693E-610.3631786
CTGTAGG41600.010.3168081
GTGCTAC2400.010.3091831
GTCCTAC40200.010.2500021
TGGCGAG38550.010.172297518
GGTATCA25750.010.1628991
TATATCG750.002635060810.1396845
AGGACGT40850.010.0993495
GGACGTG40400.010.0403996
GTCCTAA19150.09.988251
Produced by FastQC (version 0.11.5)