FastQCFastQC Report
Thu 26 May 2016
SRR1512701_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512701_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2441440
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT66370.2718477619765384No Hit
GTCCTACAGTGGACATTTCTAAATT56440.2311750442361885No Hit
GTCCTAAAGTGTGTATTTCTCATTT52990.2170440395831968No Hit
CTGTAGGACGTGGAATATGGCAAGA48620.1991447670227407No Hit
CTTTAGGACGTGAAATATGGCGAGG43750.1791975227734452No Hit
GGTATCAACGCAGAGTACTTTTTTT42750.17510157939576643No Hit
TATCAACGCAGAGTACTTTTTTTTT37840.15499049741136378No Hit
GTCCTACAGTGTGCATTTCTCATTT29200.11960154662821942No Hit
GTACATGGGAAGCAGTGGTATCAAC28890.11833180418113899No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCGT400.00532558114.2316436
TAGGACC10550.013.21991354
CGTCGGT905.5619967E-712.63758919
TAGAAAT9500.012.0844124
AATACCG550.003099074112.0753325
CCAACGA1302.7139322E-911.66546619
ATCCCGT1251.8886567E-811.3771110
AATGTCC9000.011.033598
TGTCGAG1553.274181E-1011.00308218
TGTCCAC10300.010.76959310
GTCCTAA18350.010.7548971
TCCTATA3650.010.6574852
GCGATAA1252.3012763E-710.61972811
GGCGAGG13500.010.46111519
TTTAGAA10250.010.4596792
GAAATGT10100.010.4271436
GTCCTAG4050.010.4246061
GTCCTAC28550.010.4203611
GTTTAGA2650.010.3742911
GTCCTAT3700.010.3492561
Produced by FastQC (version 0.11.5)