FastQCFastQC Report
Thu 26 May 2016
SRR1512701_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512701_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2441440
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT86760.35536404744740807No Hit
GTCCTACAGTGGACATTTCTAAATT59410.24333999606789436No Hit
GGTATCAACGCAGAGTACTTTTTTT58540.23977652532931387No Hit
TATCAACGCAGAGTACTTTTTTTTT57260.23453371780588506No Hit
GTCCTAAAGTGTGTATTTCTCATTT57080.2337964479979029No Hit
CTGTAGGACGTGGAATATGGCAAGA56750.23244478668326887No Hit
CTTTAGGACGTGAAATATGGCGAGG52410.21466839242414312No Hit
GTACATGGGAAGCAGTGGTATCAAC33020.13524805033095222No Hit
GTCCTACAGTGTGCATTTCTCATTT29500.12083032964152304No Hit
CTGAAGGACCTGGAATATGGCGAGA29280.11992922209843372No Hit
ACGCAGAGTACTTTTTTTTTTTTTT28580.11706206173405859No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC11450.012.8676454
GGCGAGG14600.011.1860519
AGGACCT22650.011.07965855
GGACCTG22400.010.5174266
GTCCTAT3100.010.4379621
TGGCGAG33000.010.32955718
TAGAAAT10200.010.1577894
GTCCTAA18000.010.151431
TTAGGAC19200.010.1486363
ACCTTTT8900.010.13525915
GACGTGA16450.010.1053487
TGTAGGA33150.010.036542
GTGTAGA3800.010.0178591
ACCGTCC951.6463955E-49.9989768
GTAGGAC32700.09.9701433
AAATGTC9150.09.9661927
TAAGACG1054.0809988E-59.9580954
AGGACGT31950.09.9074995
GTATTAA2500.09.8976451
CACCTTT9350.09.85137714
Produced by FastQC (version 0.11.5)