FastQCFastQC Report
Thu 26 May 2016
SRR1512700_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512700_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2144794
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT59590.27783554038289926No Hit
GTCCTACAGTGGACATTTCTAAATT52970.24697010528750082No Hit
GTCCTAAAGTGTGTATTTCTCATTT51420.23974330401894073No Hit
CTGTAGGACGTGGAATATGGCAAGA45740.21326057420899164No Hit
CTTTAGGACGTGAAATATGGCGAGG41960.19563650401856775No Hit
GGTATCAACGCAGAGTACTTTTTTT39660.1849128634265109No Hit
TATCAACGCAGAGTACTTTTTTTTT34270.1597822448216472No Hit
GTACATGGGAAGCAGTGGTATCAAC28570.1332062659630715No Hit
GTCCTACAGTGTGCATTTCTCATTT24870.11595519196715397No Hit
CTGAAGGACCTGGAATATGGCGAGA23390.10905476236878692No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTGT250.006077344518.9758136
AATCGCG250.006116977418.95446619
TAGGACC7700.013.1844924
TAAGCGA1251.868284E-811.3852213
AATCCCG2750.011.3726819
TAAGACG600.005932855411.0692234
CCCCGTA1053.504114E-610.84764815
CTTATAC1855.456968E-1210.7703083
GGCGAGG14200.010.61183219
CGACTAA1355.7712896E-810.54533317
TGTAGGA31800.010.5324192
GTATTAG1501.4793841E-810.4664161
ACTGTTC7800.010.42775258
GGACGTG32400.010.4249846
AGGACGT33400.010.3401145
GCTCTAG951.1860143E-410.32871
ACTATCG2300.010.30689611
GATATAC7150.010.2925861
TTAGGAC19300.010.2747093
CGCCCTA1303.8904545E-710.22158216
Produced by FastQC (version 0.11.5)