FastQCFastQC Report
Thu 26 May 2016
SRR1512700_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512700_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2144794
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT77220.3600345767472308No Hit
GTCCTACAGTGGACATTTCTAAATT56300.2624960718838266No Hit
CTGTAGGACGTGGAATATGGCAAGA56040.26128383425168106No Hit
GTCCTAAAGTGTGTATTTCTCATTT54030.2519123048647096No Hit
GGTATCAACGCAGAGTACTTTTTTT52350.24407938478007676No Hit
TATCAACGCAGAGTACTTTTTTTTT49760.2320036329829345No Hit
CTTTAGGACGTGAAATATGGCGAGG49700.23172388583705475No Hit
GTACATGGGAAGCAGTGGTATCAAC31440.14658750444098595No Hit
CTGAAGGACCTGGAATATGGCGAGA27340.12747144947253675No Hit
GTCCTACAGTGTGCATTTCTCATTT25540.11907903509614443No Hit
ACGCAGAGTACTTTTTTTTTTTTTT24890.11604844101578053No Hit
TTTCTAAATTTTCCACCTTTTTCAG22840.10649041353155596No Hit
ATTTAGAAATGTCCACTGTAGGACG22640.10555792304529014No Hit
CCCATGTACTCTGCGTTGATACCAC22450.10467205708333761No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21660.10098871966258766No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC8000.012.7143824
TCCAACG2450.012.40134218
TGGACCG853.915118E-612.3025615
CACCTTT8950.011.56455114
CCAACGT1451.2296368E-911.13154919
ACCTTTT9300.011.12880715
CGAGTAA600.005880542611.08240810
GGCGAGG13750.010.9791619
ACACCGT700.001491970310.8569976
GGACCGT803.772404E-410.6873556
TGCGCCA909.539601E-510.55319812
AATGTCC8750.010.4219888
GGTATCA21300.010.3646851
TTAGGAC19550.010.3076623
CTGTAGG30350.010.2840191
GTCCTAC26150.010.225451
AAATGTC8200.010.1942717
TGTAGGA30700.010.187062
TAGAAAT8900.010.146944
GAAATGT8350.010.1256126
Produced by FastQC (version 0.11.5)