FastQCFastQC Report
Thu 26 May 2016
SRR1512699_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512699_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2728615
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT129540.47474634567353763No Hit
CTGTAGGACGTGGAATATGGCAAGA118840.4355323121803552No Hit
GTCCTAAAGTGTGTATTTCTCATTT114410.419296969341589No Hit
CTTTAGGACGTGAAATATGGCGAGG97160.3560780835698697No Hit
GTATCAACGCAGAGTACTTTTTTTT84320.30902124337805076No Hit
GTCCTACAGTGTGCATTTCTCATTT54730.20057794888615654No Hit
CTGAAGGACCTGGAATATGGCGAGA51850.19002314360948686No Hit
GGTATCAACGCAGAGTACTTTTTTT49930.18298660675837375No Hit
TATCAACGCAGAGTACTTTTTTTTT48600.1781123390438006No Hit
GTCCTTCAGTGTGCATTTCTCATTT46660.17100250493382174No Hit
ATTTAGAAATGTCCACTGTAGGACG43880.16081418595148086No Hit
GAATATGGCAAGAAAACTGAAAATC40210.14736413894961362No Hit
TTTCTAAATTTTCCACCTTTTTCAG38020.13933808910381273No Hit
CTGTAGGACCTGGAATATGGCGAGA37850.13871506240345377No Hit
GGAATATGGCGAGAAAACTGAAAAT34630.12691420372606615No Hit
ATTCCAGGTCCTTCAGTGTGCATTT31470.11533323682527584No Hit
TTCCAGGTCCTTCAGTGTGCATTTC28250.10353237814788821No Hit
GCCATATTCCACGTCCTACAGTGGA27340.10019735286949606No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG3350.014.42398518
TAGGACC19250.013.8497994
CCAACGA2350.013.308419
ACCTTTT14850.012.46155315
GGCGAGG29600.011.91052719
GTGTAGG6800.011.6967291
CCAACGT1552.7284841E-1111.61720619
CACCTTT15650.011.581984514
AAATGTC15550.011.4098287
AGGACCT40550.011.3479855
GAAATGT16900.011.2282276
CCGGTTT2450.011.22555212
TAGAAAT17700.011.203174
AATGTCC16400.011.130298
ATTTAGA16450.011.1028721
TACGCAT600.00593381211.0691615
TTTAGAA18600.011.0181512
TGTAGGA69150.010.9491042
GGACCTG40750.010.9430446
TGCCGGT2600.010.94026110
Produced by FastQC (version 0.11.5)