FastQCFastQC Report
Thu 26 May 2016
SRR1512699_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512699_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2728615
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA137550.5041018978492752No Hit
GTCCTACAGTGGACATTTCTAAATT134060.4913115261771998No Hit
GTCCTAAAGTGTGTATTTCTCATTT118650.4348359882211305No Hit
CTTTAGGACGTGAAATATGGCGAGG114740.4205063741128741No Hit
GTATCAACGCAGAGTACTTTTTTTT106810.3914440109725996No Hit
TATCAACGCAGAGTACTTTTTTTTT71580.26233088948056066No Hit
GGTATCAACGCAGAGTACTTTTTTT69560.2549278663351187No Hit
CTGAAGGACCTGGAATATGGCGAGA63110.23128950035091064No Hit
GTCCTACAGTGTGCATTTCTCATTT58170.21318507741106751No Hit
ATTTAGAAATGTCCACTGTAGGACG49930.18298660675837375No Hit
GTCCTTCAGTGTGCATTTCTCATTT47730.17492390828313997No Hit
CTGTAGGACCTGGAATATGGCGAGA47100.17261504462886848No Hit
TTTCTAAATTTTCCACCTTTTTCAG45930.16832715498522144No Hit
GAATATGGCAAGAAAACTGAAAATC43320.15876186270323955No Hit
GGAATATGGCGAGAAAACTGAAAAT37400.13706587407897414No Hit
ATTCCAGGTCCTTCAGTGTGCATTT36880.13516014534846432No Hit
TTCCAGGTCCTTCAGTGTGCATTTC33960.12445874555406314No Hit
ACGCAGAGTACTTTTTTTTTTTTTT33240.12182004423489572No Hit
ACAGTGGACATTTCTAAATTTTCCA33220.12174674697602997No Hit
GCCATATTCCACGTCCTACAGTGGA31080.11390394027739348No Hit
ACCTGGAATATGGCGAGAAAACTGA30550.11196156291745078No Hit
GACCTGGAATATGGCGAGAAAACTG28760.10540145824896514No Hit
CCATATTCCAGGTCCTTCAGTGTGC28590.10477843154860617No Hit
GTACATGGGAAGCAGTGGTATCAAC28310.10375226992448551No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGGTA350.002159749616.2981222
TAGGACC18200.014.2060134
TCCAACG2800.013.22572318
GGCGAGG31600.011.80914219
TAGAAAT17450.011.7116394
CACCTTT14800.011.67740614
CCACCTT14750.011.5249113
AATGTCC16100.011.5046348
AAATGTC16450.011.4910377
TAAGACG1251.8182618E-811.4065934
TTTAGAA18250.011.2524012
GAAATGT17900.011.2510926
AGGACCT39300.011.2475785
ACCTTTT15550.011.23590915
ATTTAGA17100.011.1283561
GGACCTG38400.011.0088176
TGTCCAC17950.010.95393110
ATGTCCA17200.010.7688739
TAGGACT6450.010.6107844
TGTAGGA69150.010.5727632
Produced by FastQC (version 0.11.5)