FastQCFastQC Report
Thu 26 May 2016
SRR1512698_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512698_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2974461
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT89480.30082761212871845No Hit
GTATCAACGCAGAGTACTTTTTTTT85870.2886909594713126No Hit
GTCCTAAAGTGTGTATTTCTCATTT80750.27147775681039354No Hit
CTGTAGGACGTGGAATATGGCAAGA78940.2653926207134671No Hit
CTTTAGGACGTGAAATATGGCGAGG64560.2170477273025264No Hit
GTCCTACAGTGTGCATTTCTCATTT54130.18198255078819323No Hit
GGTATCAACGCAGAGTACTTTTTTT54100.1818816921788519No Hit
TATCAACGCAGAGTACTTTTTTTTT51930.17458625276982956No Hit
CTGTAGGACCTGGAATATGGCGAGA38780.13037656234188313No Hit
CTGAAGGACCTGGAATATGGCGAGA33940.11410470670148307No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAGG1700.013.38089419
TAGGACC16250.013.3702064
GGTCGGT500.001520365413.27634111
ATACGAC550.003099368712.0753223
GTGTAGG5250.011.96474551
TGTCGAG2650.011.79976718
AGGACCT31850.011.3493655
GGCGAGG22700.011.31530419
CGTGCAT855.4294665E-511.15545710
TGTAGGA51100.011.08451752
TGGACCG600.00593523611.0688575
TAGAAAT11200.011.0123844
GTATAAT4200.010.9832631
TATGTCG3050.010.8912716
ATCCTAC4700.010.8589621
CGTTTCC1752.1827873E-1110.84626614
ACCGTGC1752.1827873E-1110.8082258
TCCAACG2900.010.78254618
GTATAAC2550.010.7770691
GGACCTG31900.010.7367426
Produced by FastQC (version 0.11.5)