FastQCFastQC Report
Thu 26 May 2016
SRR1512698_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512698_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2974461
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT110770.37240360522461047No Hit
CTGTAGGACGTGGAATATGGCAAGA93080.3129306452496772No Hit
GTCCTACAGTGGACATTTCTAAATT90710.30496281511171264No Hit
GTCCTAAAGTGTGTATTTCTCATTT82020.2757474379391762No Hit
CTTTAGGACGTGAAATATGGCGAGG80030.2690571501862018No Hit
TATCAACGCAGAGTACTTTTTTTTT73820.24817941805254803No Hit
GGTATCAACGCAGAGTACTTTTTTT73510.24713721242268769No Hit
GTCCTACAGTGTGCATTTCTCATTT59060.19855698225661728No Hit
CTGTAGGACCTGGAATATGGCGAGA46320.15572569282300222No Hit
CTGAAGGACCTGGAATATGGCGAGA42310.1422442587077121No Hit
ACGCAGAGTACTTTTTTTTTTTTTT35200.11834076829381861No Hit
ATTTAGAAATGTCCACTGTAGGACG33460.1124909689520219No Hit
TTTCTAAATTTTCCACCTTTTTCAG33160.11148238285860868No Hit
GAATATGGCAAGAAAACTGAAAATC31390.10553172490747062No Hit
GTCCTTCAGTGTGCATTTCTCATTT30260.10173271728894748No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC17550.013.7040984
TCCAACG2000.013.29279918
ACCGCCC751.482273E-512.6644928
CCAACGA1251.8497303E-811.39344419
GGTATCA25650.011.280471
TGTAGGA51250.011.2785512
CACCGCC1104.9833943E-711.22553357
AGGACCT32950.011.2231785
GGCGAGG22700.011.08393119
GGACCTG31550.010.959426
CTAGGAC4200.010.8640483
GTAGGAC51300.010.7290413
ATCCCGT1605.475158E-1010.68566510
TGGCGAG53400.010.66837818
CTGTAGG51900.010.58155251
GGACGTG50700.010.3984976
AGGACGT51800.010.3507315
TTAGGAC33450.010.3159743
AATGTCC11600.010.2353118
CCACCTT13050.010.188621513
Produced by FastQC (version 0.11.5)