FastQCFastQC Report
Thu 26 May 2016
SRR1512697_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512697_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3044053
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT69040.22680288418105732No Hit
GTATCAACGCAGAGTACTTTTTTTT63410.2083078054160029No Hit
CTGTAGGACGTGGAATATGGCAAGA59360.1950031750432729No Hit
GTCCTAAAGTGTGTATTTCTCATTT58480.1921122923943834No Hit
CTTTAGGACGTGAAATATGGCGAGG46910.154103755749325No Hit
GTCCTACAGTGTGCATTTCTCATTT45890.15075295995174853No Hit
GGTATCAACGCAGAGTACTTTTTTT40410.1327506452745731No Hit
TATCAACGCAGAGTACTTTTTTTTT37600.12351953136164186No Hit
CTGTAGGACCTGGAATATGGCGAGA30550.10035961923133402No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC14700.013.5531224
CCGTGCG1201.069202E-811.8236179
GGTATCA18700.011.8114711
GATCGTC658.118678E-411.6765367
AATGCCG2000.011.36996818
GTGTAGG5650.011.2934861
AGGACCT25050.011.1346615
GGACCTG23350.011.0920846
TGTAGGA43250.010.9897792
AGGACCG1658.185452E-1110.9246385
TACGGCG700.001502983210.84769616
AATGTCC9600.010.8365318
CGTGCCG700.001516299610.8362310
GTCCTAC35800.010.8312151
GGCGAGG16650.010.70235319
GTAGGAC43350.010.5923823
TCCTACA39850.010.5704152
GTCGTAC750.002017279910.4710881
TTAGGAC20600.010.2701093
CTGTAGG39950.010.246661
Produced by FastQC (version 0.11.5)