FastQCFastQC Report
Thu 26 May 2016
SRR1512697_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512697_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3044053
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT82500.2710202483333897No Hit
GTCCTACAGTGGACATTTCTAAATT71280.23416149456004873No Hit
CTGTAGGACGTGGAATATGGCAAGA67530.22184239236307646No Hit
GTCCTAAAGTGTGTATTTCTCATTT60020.19717133702994No Hit
TATCAACGCAGAGTACTTTTTTTTT56580.18587061394791746No Hit
CTTTAGGACGTGAAATATGGCGAGG56150.18445802356266464No Hit
GGTATCAACGCAGAGTACTTTTTTT55280.18159999185296707No Hit
GTCCTACAGTGTGCATTTCTCATTT48380.15893284381053813No Hit
CTGTAGGACCTGGAATATGGCGAGA37130.12197553721962134No Hit
CTGAAGGACCTGGAATATGGCGAGA31310.1028562906099204No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG2200.012.51596118
CCAACGA1750.012.47896819
TAGGACC13550.012.4161454
GGTATCA21300.012.3289261
TAGGCCG657.9874287E-411.6992495
GCACCGT1201.2752753E-711.0842646
ACGGCGT600.005879932511.0828078
CGTGCCG600.00588137111.08244110
GGCGAGG15850.010.90262319
CGTTTCC1803.6379788E-1110.55106514
AGGACCT25700.010.3933275
TGTAGGA40400.010.1647812
TTAGGAC24450.010.1461243
TAGGACA11100.010.104434
TAGACAG4150.010.0782695
TAAGACG856.56488E-410.0641344
TTAGGCA2002.5465852E-119.9802674
GTCCTAC38400.09.9607171
TGGACCG1054.0810206E-59.958295
TGGCGAG39150.09.9435418
Produced by FastQC (version 0.11.5)