FastQCFastQC Report
Thu 26 May 2016
SRR1512696_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512696_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2336973
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT83360.3567007406589635No Hit
GTCCTACAGTGGACATTTCTAAATT76070.3255065420096852No Hit
GTACATGGGAAGCAGTGGTATCAAC71130.304368086409214No Hit
CTGTAGGACGTGGAATATGGCAAGA68680.2938844394008831No Hit
GTCCTAAAGTGTGTATTTCTCATTT68590.29349932583731175No Hit
CTTTAGGACGTGAAATATGGCGAGG54530.23333602912827836No Hit
GGTATCAACGCAGAGTACTTTTTTT51030.21835939054494852No Hit
CCCATGTACTCTGCGTTGATACCAC48950.20945898818685538No Hit
TATCAACGCAGAGTACTTTTTTTTT48020.2054794813632849No Hit
GTCCTACAGTGTGCATTTCTCATTT37110.1587951593792483No Hit
CTGAAGGACCTGGAATATGGCGAGA31390.13431905289449214No Hit
GAGTACATGGGAAGCAGTGGTATCA29970.1282428166692555No Hit
GTCCTTCAGTGTGCATTTCTCATTT28080.12015543183425739No Hit
GTACTTTTTTTTTTTTTTTTTTTTT26590.11377966283735413No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25790.11035643116116448No Hit
ATTTAGAAATGTCCACTGTAGGACG25320.10834528255140304No Hit
GAATATGGCAAGAAAACTGAAAATC23820.10192672315854741No Hit
TTTCTAAATTTTCCACCTTTTTCAG23590.10094254405164287No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAGG1400.014.89195919
TAGGACC9950.013.1582784
AGGACCG951.0574295E-611.983975
CGTGCGC1302.6993803E-911.66965510
TGTCGAG1900.011.46883618
CCGTGCG752.1583744E-411.3515389
GCGTGCG1251.9523213E-811.3515389
CCACCTT9550.011.32589713
GGCGAGG16550.011.28042419
AATGTCC10300.011.2014678
GTGTAGG4950.011.1107891
ACGACTG600.005935902211.0685285
TATGTCG2350.010.90453916
AAATGTC10450.010.89475257
TAGAAAT10450.010.8945194
CACCTTT10200.010.88648714
AGAAACG700.001508611110.842645
AAGGCGT1408.532879E-910.842646
GAAATGT10250.010.8294176
TAGGACT3400.010.6034644
Produced by FastQC (version 0.11.5)