FastQCFastQC Report
Thu 26 May 2016
SRR1512696_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512696_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2336973
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT104890.44882846314441804No Hit
GTCCTACAGTGGACATTTCTAAATT79370.3396273726739676No Hit
GTACATGGGAAGCAGTGGTATCAAC79280.33924225911039624No Hit
CTGTAGGACGTGGAATATGGCAAGA77930.3334655556568262No Hit
GTCCTAAAGTGTGTATTTCTCATTT72620.31074385540611726No Hit
GGTATCAACGCAGAGTACTTTTTTT71010.3038546016577855No Hit
TATCAACGCAGAGTACTTTTTTTTT70490.30162950106826225No Hit
CTTTAGGACGTGAAATATGGCGAGG68250.29204445237493115No Hit
CCCATGTACTCTGCGTTGATACCAC58580.2506661394889885No Hit
CTGAAGGACCTGGAATATGGCGAGA38450.164529072436866No Hit
GTCCTACAGTGTGCATTTCTCATTT37770.16161932551210478No Hit
GAGTACATGGGAAGCAGTGGTATCA34670.1483543027668698No Hit
ACGCAGAGTACTTTTTTTTTTTTTT34310.14681384851258444No Hit
GTACTTTTTTTTTTTTTTTTTTTTT31390.13431905289449214No Hit
ATTTAGAAATGTCCACTGTAGGACG29930.128071655085446No Hit
TTTCTAAATTTTCCACCTTTTTCAG29470.12610329687163696No Hit
GTCCTTCAGTGTGCATTTCTCATTT28800.1232363403428281No Hit
GAATATGGCAAGAAAACTGAAAATC27400.11724568490949618No Hit
CTGTAGGACCTGGAATATGGCGAGA25690.10992852720164076No Hit
GAGTACTTTTTTTTTTTTTTTTTTT24060.1029536926614043No Hit
GGAATATGGCGAGAAAACTGAAAAT23820.10192672315854741No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACGC250.006013049719.010883
CGCGCGA400.00529386714.24443113
TAGGACC12000.013.3079014
AGGACCT25550.011.5337615
ATTCGGA752.0776647E-411.39822811
TACCGTA752.0776647E-411.3982287
CCAACGA1251.8473656E-811.39408219
GGCGAGG18250.011.34205319
CGTGCGC855.333725E-511.17473310
GTCGAGG1451.2296368E-911.13214819
TCCAACG1658.185452E-1110.93371518
AATCGCC700.00149836510.85150618
TGTAGGA43000.010.81222252
TAGAAAT11000.010.7154534
CACCTTT10150.010.66532514
CCCGTTT1701.4006218E-1010.61531512
AATGTCC10700.010.5637788
GGACCTG25600.010.53816
GCGTGCG1002.4039118E-510.4483759
GTAGGAC43800.010.4386223
Produced by FastQC (version 0.11.5)