##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512696_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2336973 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.74904031839478 33.0 31.0 34.0 30.0 34.0 2 31.87329079112168 34.0 31.0 34.0 30.0 34.0 3 31.973888444581945 34.0 31.0 34.0 30.0 34.0 4 35.41389994664038 37.0 35.0 37.0 33.0 37.0 5 35.201594113410806 37.0 35.0 37.0 33.0 37.0 6 35.05929636328704 37.0 35.0 37.0 32.0 37.0 7 35.056110190404425 37.0 35.0 37.0 32.0 37.0 8 35.058267254264386 37.0 35.0 37.0 32.0 37.0 9 36.66447494258598 39.0 37.0 39.0 33.0 39.0 10 36.46467716999726 39.0 37.0 39.0 32.0 39.0 11 36.57879145373096 39.0 37.0 39.0 32.0 39.0 12 36.529354425575306 39.0 37.0 39.0 32.0 39.0 13 36.52994364932757 39.0 37.0 39.0 32.0 39.0 14 37.789165728487234 40.0 38.0 41.0 32.0 41.0 15 37.78092344241889 40.0 38.0 41.0 32.0 41.0 16 37.74286438054697 40.0 38.0 41.0 32.0 41.0 17 37.74022806425235 40.0 38.0 41.0 32.0 41.0 18 37.67848665774059 40.0 38.0 41.0 32.0 41.0 19 37.692118822083096 40.0 38.0 41.0 32.0 41.0 20 37.66421092584296 40.0 38.0 41.0 32.0 41.0 21 37.61789802449579 40.0 38.0 41.0 32.0 41.0 22 37.53166425114882 40.0 38.0 41.0 32.0 41.0 23 37.479069719675834 40.0 38.0 41.0 32.0 41.0 24 37.44907108468947 40.0 38.0 41.0 32.0 41.0 25 37.380065152656876 40.0 38.0 41.0 32.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.552612602131628 1101 2 0.5293461247474518 1101 3 0.553077734917558 1101 4 0.4920113174380063 1101 5 0.528871048164909 1101 6 0.7144395533273027 1101 7 0.6521078244729921 1101 8 0.7092038003232943 1101 9 0.7662913937497748 1101 10 0.8106438955649082 1101 11 0.8207235829774149 1101 12 0.7724779498111118 1101 13 0.8310852145359817 1101 14 0.9060558712649325 1101 15 0.8516997522019167 1101 16 0.9565207600644996 1101 17 0.9460463574579592 1101 18 1.0115765200552929 1101 19 1.0015124095844925 1101 20 1.03496039516358 1101 21 1.026990894661786 1101 22 0.9935408438700222 1101 23 0.956213602437451 1101 24 0.9078851276985347 1101 25 1.0504473555777807 1102 1 0.5128798627106939 1102 2 0.5211087718998506 1102 3 0.5196563752823948 1102 4 0.4920611636571195 1102 5 0.5797894912659771 1102 6 0.6983530118064607 1102 7 0.6276407547943421 1102 8 0.5602569342717487 1102 9 0.5584655373493064 1102 10 0.7840907085885576 1102 11 0.6586565557210804 1102 12 0.6739309140703114 1102 13 0.6534460163177229 1102 14 0.7660982935790699 1102 15 0.6255474560941607 1102 16 0.7668580174618924 1102 17 0.8396808892216612 1102 18 0.7509826076573702 1102 19 0.8324478216112183 1102 20 0.8812548060236907 1102 21 0.8505416423550756 1102 22 0.7987916658023622 1102 23 0.8500814569289119 1102 24 0.9520859973559723 1102 25 0.867578546584248 1103 1 0.5027776759804574 1103 2 0.447403714421025 1103 3 0.4509648723963551 1103 4 0.4441866515160342 1103 5 0.47597253341582757 1103 6 0.6443712897056031 1103 7 0.518000836760443 1103 8 0.4537290578633204 1103 9 0.6294120755585553 1103 10 0.7270176492366645 1103 11 0.616595909318896 1103 12 0.6035151431419621 1103 13 0.7021190981154817 1103 14 0.6675245734286861 1103 15 0.7442854954479543 1103 16 0.7018242228918581 1103 17 0.674316598656084 1103 18 0.6674178174778191 1103 19 0.7953525485940531 1103 20 0.8591584010383926 1103 21 0.8155945933089228 1103 22 0.7389502187407331 1103 23 0.7340473701756522 1103 24 0.7115270953457156 1103 25 0.711602045081726 1104 1 0.3763295671785194 1104 2 0.362761055937316 1104 3 0.30475597142955735 1104 4 0.3061910762980702 1104 5 0.33595788289880346 1104 6 0.47519707372676834 1104 7 0.42393829579195597 1104 8 0.37206094318043625 1104 9 0.3493616653366658 1104 10 0.5097633694414156 1104 11 0.46616628179607034 1104 12 0.41496972913153485 1104 13 0.4379735350815963 1104 14 0.42150587735432765 1104 15 0.3711602320460301 1104 16 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-2.1535717266343184 2314 17 -2.238645933659676 2314 18 -1.9467411502080836 2314 19 -1.89917328481161 2314 20 -2.1066226522565685 2314 21 -1.932089210753162 2314 22 -1.8561820275270549 2314 23 -1.894316621486439 2314 24 -2.0558977108806857 2314 25 -2.0655884788743464 2315 1 -0.6597018725193635 2315 2 -0.654794738063508 2315 3 -0.7002153618950082 2315 4 -0.6668764051749179 2315 5 -0.7183634782687349 2315 6 -0.5360002493388052 2315 7 -0.848521964547686 2315 8 -0.8744489759966427 2315 9 -1.0534353477918117 2315 10 -0.930394906436419 2315 11 -0.8571977633715591 2315 12 -1.0471224840435482 2315 13 -1.0769529926646015 2315 14 -1.305148775178509 2315 15 -1.2498680725767812 2315 16 -1.1734246582203767 2315 17 -1.2068272298902798 2315 18 -0.9628736391413781 2315 19 -1.0096485015102914 2315 20 -0.9778596601179004 2315 21 -1.0743442580620481 2315 22 -1.0741548280798767 2315 23 -1.0797991332960777 2315 24 -1.0547953243500316 2315 25 -1.1646966211900036 2316 1 -1.411573886926412 2316 2 -1.3145725921208395 2316 3 -1.3505818183423735 2316 4 -1.0615417612625464 2316 5 -1.2551038510240744 2316 6 -4.891836555593908 2316 7 -2.5541583166171264 2316 8 -1.798728780891878 2316 9 -1.8223547935548368 2316 10 -1.5599704852968515 2316 11 -1.6619672783338117 2316 12 -1.6842752557527518 2316 13 -1.7877920452166336 2316 14 -1.9614870583712403 2316 15 -2.0831835788659987 2316 16 -2.0412260570258027 2316 17 -2.1171805013159997 2316 18 -2.343444095231739 2316 19 -2.1681260299968557 2316 20 -2.390000413136903 2316 21 -2.582979654928174 2316 22 -2.4953228233856493 2316 23 -2.500697276090932 2316 24 -2.5031143739099164 2316 25 -2.4960494048771196 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 21376.0 3 1.0 4 454.0 5 3557.0 6 4630.0 7 3614.0 8 2918.0 9 2574.0 10 2415.0 11 2183.0 12 2146.0 13 2062.0 14 2079.0 15 2100.0 16 2189.0 17 2268.0 18 2575.0 19 2887.0 20 3685.0 21 4708.0 22 5928.0 23 7632.0 24 9153.0 25 11269.0 26 12604.0 27 14654.0 28 17073.0 29 20641.0 30 26632.0 31 34822.0 32 45634.0 33 60469.0 34 80956.0 35 110677.0 36 166573.0 37 302194.0 38 1339641.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.23605439174001 19.513630291651257 13.19366975390519 25.05664556270354 2 14.639918787426431 20.684255306036505 41.491981456690624 23.18384444984644 3 18.86731254490317 25.47282317767471 30.02276876968626 25.637095507735864 4 12.613232903430932 17.45398900331937 37.795451812603694 32.13732628064601 5 12.653347428731998 38.50215543089771 35.79458812080008 13.049909019570215 6 31.399749736615558 36.991590961921524 18.246666543946567 13.361992757516356 7 27.49150689528388 31.777544646315746 22.59411969402994 18.136828764370435 8 24.90847633482445 37.69114246103101 20.64297916218126 16.757402041963275 9 26.799581728038476 14.595303043857347 20.639144554224902 37.965970673879276 10 15.882210431337501 28.84112610368331 34.2379812589994 21.038682205979793 11 33.54037373432108 21.978487203875865 25.54884255711875 18.932296504684306 12 24.34559870692847 24.60838227796843 31.948301109991984 19.097717905111118 13 29.590813023927875 22.214798766327203 25.747013188451824 22.447375021293098 14 20.51063024863043 23.503935710522747 27.603168768008985 28.38226527283784 15 22.8795401745998 32.403021626634064 23.199025027739893 21.51841317102624 16 20.929645413500246 27.750973408687013 29.815511292567777 21.50386988524497 17 21.233838339534476 29.332951066247027 28.479268785824168 20.953941808394326 18 21.24618193052889 26.4232231053432 31.949485518080685 20.381109446047223 19 23.59381465444386 26.50017565683031 28.397568960817615 21.508440727908212 20 23.99449885728052 27.024791514136474 28.878175106302223 20.10253452228078 21 22.152759146126208 26.50385776814709 28.13181838215504 23.21156470357167 22 23.278089033027193 28.739260053582594 27.40076501324588 20.581885900144332 23 21.0697342245717 28.550394035361126 29.099437605826 21.280434134241176 24 23.16800094481356 26.991885643326892 29.365686921820526 20.474426490039022 25 22.06636533669837 27.454660366208767 29.016081914510778 21.462892382582083 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 359.0 1 359.0 2 689.0 3 1019.0 4 1019.0 5 1019.0 6 2546.5 7 4074.0 8 4074.0 9 4074.0 10 4972.5 11 5871.0 12 5871.0 13 5871.0 14 7951.0 15 10031.0 16 10031.0 17 10031.0 18 18749.0 19 27467.0 20 27467.0 21 27467.0 22 44599.0 23 61731.0 24 61731.0 25 61731.0 26 92617.0 27 123503.0 28 123503.0 29 123503.0 30 159526.5 31 195550.0 32 195550.0 33 195550.0 34 215515.5 35 235481.0 36 235481.0 37 235481.0 38 245551.0 39 255621.0 40 255621.0 41 255621.0 42 267107.0 43 278593.0 44 278593.0 45 278593.0 46 302275.5 47 325958.0 48 325958.0 49 325958.0 50 304918.5 51 283879.0 52 283879.0 53 283879.0 54 244347.5 55 204816.0 56 204816.0 57 204816.0 58 173373.0 59 141930.0 60 141930.0 61 141930.0 62 117616.0 63 93302.0 64 93302.0 65 93302.0 66 72063.0 67 50824.0 68 50824.0 69 50824.0 70 37066.0 71 23308.0 72 23308.0 73 23308.0 74 16222.5 75 9137.0 76 9137.0 77 9137.0 78 6165.5 79 3194.0 80 3194.0 81 3194.0 82 2085.5 83 977.0 84 977.0 85 977.0 86 624.5 87 272.0 88 272.0 89 272.0 90 167.0 91 62.0 92 62.0 93 62.0 94 37.0 95 12.0 96 12.0 97 12.0 98 7.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1542165870123446 2 0.01664546402547227 3 0.0 4 0.0036799740519038943 5 0.10291090226545194 6 0.04424526941475148 7 0.028498403704278995 8 0.02961095399904064 9 0.02969653479094538 10 0.03016722914642146 11 0.03744159645832451 12 0.035516028640467816 13 0.022892861834518413 14 0.012494795618092294 15 0.0036799740519038943 16 0.003508812468094411 17 0.0016260350461900928 18 0.002524633361189881 19 0.001283711878571126 20 1.7116158380948345E-4 21 0.0 22 8.558079190474174E-4 23 0.0 24 1.7116158380948345E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2336973.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.33773317536416 #Duplication Level Percentage of deduplicated Percentage of total 1 78.52048319181912 46.5922748043683 2 13.989332324785082 16.60190537579188 3 3.825805827957976 6.810439362003706 4 1.487234855792346 3.529965801684297 5 0.6990380018181305 2.0739665215661973 6 0.3833152659377399 1.3647035383354418 7 0.22242789997234813 0.9238857165521048 8 0.15870276576648712 0.7533649895395305 9 0.11471474283038245 0.6126221521214776 >10 0.5116224891816785 5.358148429478906 >50 0.043379203263568214 1.8104156287358644 >100 0.03391961666891459 4.233050755989118 >500 0.0057691017014837006 2.3581942192807204 >1k 0.0036056885634267455 4.056941312570488 >5k 5.769101701482793E-4 2.471289979061939 >10k+ 7.211377126853491E-5 0.44883141292020634 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 10489 0.44882846314441804 No Hit GTCCTACAGTGGACATTTCTAAATT 7937 0.3396273726739676 No Hit GTACATGGGAAGCAGTGGTATCAAC 7928 0.33924225911039624 No Hit CTGTAGGACGTGGAATATGGCAAGA 7793 0.3334655556568262 No Hit GTCCTAAAGTGTGTATTTCTCATTT 7262 0.31074385540611726 No Hit GGTATCAACGCAGAGTACTTTTTTT 7101 0.3038546016577855 No Hit TATCAACGCAGAGTACTTTTTTTTT 7049 0.30162950106826225 No Hit CTTTAGGACGTGAAATATGGCGAGG 6825 0.29204445237493115 No Hit CCCATGTACTCTGCGTTGATACCAC 5858 0.2506661394889885 No Hit CTGAAGGACCTGGAATATGGCGAGA 3845 0.164529072436866 No Hit GTCCTACAGTGTGCATTTCTCATTT 3777 0.16161932551210478 No Hit GAGTACATGGGAAGCAGTGGTATCA 3467 0.1483543027668698 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 3431 0.14681384851258444 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 3139 0.13431905289449214 No Hit ATTTAGAAATGTCCACTGTAGGACG 2993 0.128071655085446 No Hit TTTCTAAATTTTCCACCTTTTTCAG 2947 0.12610329687163696 No Hit GTCCTTCAGTGTGCATTTCTCATTT 2880 0.1232363403428281 No Hit GAATATGGCAAGAAAACTGAAAATC 2740 0.11724568490949618 No Hit CTGTAGGACCTGGAATATGGCGAGA 2569 0.10992852720164076 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 2406 0.1029536926614043 No Hit GGAATATGGCGAGAAAACTGAAAAT 2382 0.10192672315854741 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.7116158380948347E-4 0.0 5 0.0 0.0 0.0 1.7116158380948347E-4 0.0 6 0.0 0.0 0.0 1.7116158380948347E-4 0.0 7 0.0 0.0 0.0 1.7116158380948347E-4 0.0 8 0.0 0.0 0.0 1.7116158380948347E-4 0.0 9 0.0 0.0 0.0 1.7116158380948347E-4 0.0 10 0.0 0.0 0.0 2.1395197976185434E-4 0.0 11 0.0 0.0 0.0 2.567423757142252E-4 0.0 12 0.0 0.0 0.0 2.567423757142252E-4 0.0 13 0.0 0.0 0.0 2.9953277166659605E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATTACGC 25 0.0060130497 19.01088 3 CGCGCGA 40 0.005293867 14.244431 13 TAGGACC 1200 0.0 13.307901 4 AGGACCT 2555 0.0 11.533761 5 ATTCGGA 75 2.0776647E-4 11.398228 11 TACCGTA 75 2.0776647E-4 11.398228 7 CCAACGA 125 1.8473656E-8 11.394082 19 GGCGAGG 1825 0.0 11.342053 19 CGTGCGC 85 5.333725E-5 11.174733 10 GTCGAGG 145 1.2296368E-9 11.132148 19 TCCAACG 165 8.185452E-11 10.933715 18 AATCGCC 70 0.001498365 10.851506 18 TGTAGGA 4300 0.0 10.8122225 2 TAGAAAT 1100 0.0 10.715453 4 CACCTTT 1015 0.0 10.665325 14 CCCGTTT 170 1.4006218E-10 10.615315 12 AATGTCC 1070 0.0 10.563778 8 GGACCTG 2560 0.0 10.5381 6 GCGTGCG 100 2.4039118E-5 10.448375 9 GTAGGAC 4380 0.0 10.438622 3 >>END_MODULE