FastQCFastQC Report
Thu 26 May 2016
SRR1512695_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512695_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2366165
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT61810.26122438629596834No Hit
GGTATCAACGCAGAGTACTTTTTTT39490.1668945318690793No Hit
TATCAACGCAGAGTACTTTTTTTTT36680.1550187751065543No Hit
GTACATGGGAAGCAGTGGTATCAAC26640.11258724560628697No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCC1051.4642865E-913.5128718
GTATTAG1900.012.3940081
TCCGGAC550.003099508412.0750813
TAGGACC6050.011.6046234
TAAGACG600.005934758611.0688244
CGTCGGT803.8558192E-410.66217619
CCGTCCG803.918295E-410.6436419
GTCTTAC1708.913048E-1010.3890951
AGGACCG1106.143595E-610.3500695
GTATAAT2850.010.328341
GTCCTAG3050.010.2944761
TGGCGAG11000.010.16395618
GTATTAA2800.010.1623491
TATGGCG11650.010.10200116
GCGTCGG856.758156E-410.03221918
GGACCGA951.666518E-49.9869096
GGTATCA22350.09.9216761
GGACCGT1356.56235E-79.8389556
ATGGCGA11700.09.81484517
ACTATAC1451.649114E-79.814723
Produced by FastQC (version 0.11.5)