FastQCFastQC Report
Thu 26 May 2016
SRR1512695_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512695_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2366165
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT75100.31739122166036604No Hit
GGTATCAACGCAGAGTACTTTTTTT51600.21807439464280812No Hit
TATCAACGCAGAGTACTTTTTTTTT51400.21722914505116928No Hit
GTACATGGGAAGCAGTGGTATCAAC29710.12556182683794243No Hit
ACGCAGAGTACTTTTTTTTTTTTTT24000.10142995099665492No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCGT1103.819696E-812.090336
TATCGCG550.00307230512.0893097
ACCGTGC1354.7421054E-911.2577698
GTATTAC1605.2568794E-1010.7096311
GTATTAA2050.010.6806081
GTACTAG909.321404E-510.5774141
GGACCGT1355.695256E-810.5550516
GTACCGT1451.4455509E-810.4822586
TAGGACC5550.010.2762974
TCTATAC2800.010.1845453
AATCCCG1409.773248E-810.17292319
TAAACCT2201.8189894E-129.9376464
GTAGGAC11650.09.8727423
GGACGTG11400.09.8328636
GTATTAG1659.886207E-99.8081471
GACGTGA6350.09.7231227
TAGGACG11400.09.67233854
GTCCTAA5350.09.6086411
GAATAAC1401.0295626E-69.5196721
GGTCTAC3000.09.5196721
Produced by FastQC (version 0.11.5)