FastQCFastQC Report
Thu 26 May 2016
SRR1512694_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512694_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2694697
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT68540.2543514168754409No Hit
GTCCTACAGTGGACATTTCTAAATT58230.21609108556546433No Hit
GTCCTAAAGTGTGTATTTCTCATTT52210.1937509115125003No Hit
CTGTAGGACGTGGAATATGGCAAGA49600.18406522143305906No Hit
CTTTAGGACGTGAAATATGGCGAGG43390.1610199588302507No Hit
GGTATCAACGCAGAGTACTTTTTTT41840.15526792066046757No Hit
TATCAACGCAGAGTACTTTTTTTTT40640.15081472981934518No Hit
GTCCTACAGTGTGCATTTCTCATTT34430.1277694672165368No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC10100.012.5871814
GTACTAA1458.683855E-910.8307091
TCGCATT700.001524495710.829144518
TCCGCGT700.001546921810.81028
GTGTAGG4850.010.7260311
TAGAAAT8950.010.6003934
GACCGTC909.649486E-510.5415037
GTAGGAC34450.010.5200833
GGCGAGG15500.010.51749619
GACGTGG16150.010.5153947
AAATGTC8950.010.494397
TGTAGGA35900.010.4651582
GAAATGT8450.010.4417026
GTCCTAT3600.010.3606271
TAGGACA9800.010.358644
AGGACGT33800.010.2732875
GTCCTAC29600.010.146931
AATGTCC8950.010.0401988
GGACGTG34050.010.0306826
ATAGGAC2300.09.8998473
Produced by FastQC (version 0.11.5)