FastQCFastQC Report
Thu 26 May 2016
SRR1512694_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512694_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2694697
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT90690.3365498978178252No Hit
GTCCTACAGTGGACATTTCTAAATT61940.22985886724926774No Hit
TATCAACGCAGAGTACTTTTTTTTT60170.2232904107586122No Hit
GGTATCAACGCAGAGTACTTTTTTT59630.2212864748801071No Hit
CTGTAGGACGTGGAATATGGCAAGA58920.2186516702991097No Hit
GTCCTAAAGTGTGTATTTCTCATTT54780.20328816189723742No Hit
CTTTAGGACGTGAAATATGGCGAGG51950.19278605349692376No Hit
GTCCTACAGTGTGCATTTCTCATTT36710.13623052981466935No Hit
CTGTAGGACCTGGAATATGGCGAGA29080.10791565804986608No Hit
ACGCAGAGTACTTTTTTTTTTTTTT28890.10721056950002171No Hit
CTGAAGGACCTGGAATATGGCGAGA27160.10079055270407024No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC10050.013.9052114
TATACCG551.9494326E-413.8278165
GCGAATT550.003078212.08631413
GTACTAG802.812264E-511.897871
TGTAGGA34950.011.3989742
GGCGAGG15250.011.20656719
ACCGACC600.005885252311.0813858
CTGTAGG34800.010.9405691
GTAGGAC34800.010.7359293
GTATTAG1605.275069E-1010.7080831
AGGACGT35300.010.6377245
TGGCGAG32050.010.60554318
AAGTCGT2600.010.5944877
TTAGGAC20400.010.5784483
GGACGTG34700.010.4845256
AGTCGTC2900.010.4809158
GGTATCA24200.010.3442641
GTCCTAA15700.010.3064341
AGGACCT21400.010.3062465
GTCCTAC32550.010.1762421
Produced by FastQC (version 0.11.5)