FastQCFastQC Report
Thu 26 May 2016
SRR1512693_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512693_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2935911
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT71650.24404690741647142No Hit
GTCCTACAGTGGACATTTCTAAATT55440.1888340620679578No Hit
CTGTAGGACGTGGAATATGGCAAGA50190.17095204861455268No Hit
GTCCTAAAGTGTGTATTTCTCATTT49650.16911275580220245No Hit
GGTATCAACGCAGAGTACTTTTTTT45050.15344470591921894No Hit
TATCAACGCAGAGTACTTTTTTTTT41720.14210240024305915No Hit
CTTTAGGACGTGAAATATGGCGAGG41330.1407740221008062No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC10300.011.6972234
TATGTCG1604.7293724E-1111.27031116
AGGACCT20950.010.86784655
ACGACTG700.001509588310.841975
TCGCGCG1053.63847E-610.8149
GGCGAGG13550.010.63228219
GGTATCA21900.010.618791
GGACGTA909.656642E-510.5408066
GTCCTAA15000.010.4664541
TTAGGAC20050.010.3620583
GACCGTG1752.419256E-1010.2998727
GGACCTG21450.010.1722456
TGGCGAG31050.010.10233318
GTTCTAA3800.010.0705181
TTTAGGA22150.010.0220932
ACTGCGC856.857585E-410.0164578
ACCGTCC1054.3025E-59.9104628
ATCGCGC1151.0799526E-59.871298
AGGACGT33150.09.8444455
TGTAGGA33250.09.8433692
Produced by FastQC (version 0.11.5)