FastQCFastQC Report
Thu 26 May 2016
SRR1512693_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512693_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2935911
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT90330.3076728143325871No Hit
GGTATCAACGCAGAGTACTTTTTTT61340.20893003909178445No Hit
TATCAACGCAGAGTACTTTTTTTTT60800.20709074627943422No Hit
CTGTAGGACGTGGAATATGGCAAGA60530.2061710998732591No Hit
GTCCTACAGTGGACATTTCTAAATT58190.19820083101974142No Hit
GTCCTAAAGTGTGTATTTCTCATTT53850.18341836656492652No Hit
CTTTAGGACGTGAAATATGGCGAGG48970.1667966092977614No Hit
ACGCAGAGTACTTTTTTTTTTTTTT29630.10092267783321769No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAA250.005979800619.0293371
ATAACGC500.001492706413.3087243
TAGGACC9600.013.2691154
CGTCAAT658.0403866E-411.69001112
GTCCTAG3600.011.6290391
CGGTTAG600.00581064911.1004481
TGTAGGA31150.010.7747332
GGTATCA26750.010.6706571
CTAGGAC3950.010.589223
AGGACCT19950.010.4834215
CTGTAGG32350.010.4117241
GGCGAGG15350.010.39145819
GTAGGAC31850.010.237483
GTCCTAA15600.010.18557551
TATACCG750.002633653610.14032655
CGAACCG1502.4549081E-810.13134412
AAACCCG856.5549527E-410.0657665
CTAATAC1909.822543E-1110.0065613
AGGACGT33650.09.8314465
GGACCTG20200.09.8291216
Produced by FastQC (version 0.11.5)