FastQCFastQC Report
Thu 26 May 2016
SRR1512692_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512692_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2259706
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT68530.30326954037383624No Hit
GTCCTACAGTGGACATTTCTAAATT53960.2387921260553364No Hit
GTCCTAAAGTGTGTATTTCTCATTT50430.22317062485119743No Hit
CTGTAGGACGTGGAATATGGCAAGA45460.20117661324083755No Hit
GGTATCAACGCAGAGTACTTTTTTT44720.19790185094875173No Hit
CTTTAGGACGTGAAATATGGCGAGG43160.190998298008679No Hit
TATCAACGCAGAGTACTTTTTTTTT43110.19077703028624077No Hit
GTCCTACAGTGTGCATTTCTCATTT31590.13979694703647289No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC11400.012.3162994
GTCTAAG1850.012.1979471
GTATTAG1551.4551915E-1112.0268691
GGCGAGG13400.011.88500419
AATGTCC7650.011.1273518
GACGTTA600.00593780511.0680257
AAATGTC7200.010.9362647
GGTATCA18300.010.8836761
TAGAAAT7850.010.7558244
TGTAGGA32300.010.7202282
AGGACCT20950.010.5963235
GGACCTG20050.010.5514926
GTAGGAC32600.010.4760743
GGTCTAC2750.010.346561
GTGTAGG4400.010.2573641
GAAATGT8150.010.1270986
GGACGTG31850.010.0974946
GTACTAA2052.1827873E-1110.0506951
AACCGTG856.6797057E-410.044937
AGGACGT32800.010.0075015
Produced by FastQC (version 0.11.5)