FastQCFastQC Report
Thu 26 May 2016
SRR1512692_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512692_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2259706
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT87080.3853598653984191No Hit
TATCAACGCAGAGTACTTTTTTTTT61890.2738851868340395No Hit
GGTATCAACGCAGAGTACTTTTTTT61540.2723363127769719No Hit
GTCCTACAGTGGACATTTCTAAATT54990.2433502411375639No Hit
CTGTAGGACGTGGAATATGGCAAGA54000.238969140233287No Hit
CTTTAGGACGTGAAATATGGCGAGG53640.23737601263173172No Hit
GTCCTAAAGTGTGTATTTCTCATTT53590.2371547449092935No Hit
GTCCTACAGTGTGCATTTCTCATTT33560.1485148953005391No Hit
ACGCAGAGTACTTTTTTTTTTTTTT28330.12537029153350038No Hit
CTGAAGGACCTGGAATATGGCGAGA27590.12209552924141459No Hit
GTACTTTTTTTTTTTTTTTTTTTTT25330.11209422818720664No Hit
CTGTAGGACCTGGAATATGGCGAGA24770.10961602969589848No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC11750.012.3772524
GGTATCA21000.011.6431231
GGCGAGG15450.011.24612419
AAATGTC9250.010.8856887
AGACCGT909.517076E-510.5557166
TGGCGAG33150.010.54009518
GTCCTAT3000.010.4652181
TAGGACT3550.010.4425574
AGGACCT20500.010.3870845
GACGTGA19550.010.3496237
TATACTG2950.010.3116335
TAGAAAT10150.010.3014244
GTCCAAG2500.010.2749421
CCACCTT8400.010.175796513
GGACGTG33600.010.1504526
GGACCTG19950.010.1430116
GTTGCAC750.002635899710.1390993
AGGACGT34300.010.0603265
AATGTCC9850.010.0297218
TGTAGGA35450.010.0023412
Produced by FastQC (version 0.11.5)