FastQCFastQC Report
Thu 26 May 2016
SRR1512691_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512691_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2383628
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT66820.2803289775082353No Hit
GTCCTACAGTGGACATTTCTAAATT51960.2179870348896724No Hit
GTCCTAAAGTGTGTATTTCTCATTT51480.21597329784681166No Hit
CTGTAGGACGTGGAATATGGCAAGA46240.1939900017955822No Hit
GGTATCAACGCAGAGTACTTTTTTT42670.1790128325393056No Hit
TATCAACGCAGAGTACTTTTTTTTT39870.16726603312261812No Hit
CTTTAGGACGTGAAATATGGCGAGG39050.1638258990077311No Hit
GTACATGGGAAGCAGTGGTATCAAC30310.12715910368564223No Hit
GTCCTACAGTGTGCATTTCTCATTT29870.1253131780630199No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC9000.012.6482434
ATAAGAC2150.012.3538373
GCGCAGA1551.4551915E-1112.0302461
GTATAAG2550.011.9309251
GGCGAGG12800.011.70111319
ACCGAGC752.1600469E-411.3506118
AATGTCC8200.011.304478
ATTCGTT600.0059617711.062077510
TACGACA700.001510127910.8413514
TAAGACT2800.010.8413514
CTGACGA700.001543937110.8126089
AAATGTC8400.010.728427
GTATAAA3450.010.5253171
CACCTTT8600.010.48467314
GTGTAGG4400.010.48329451
TAGAAAT8850.010.3972844
AGGACGT30450.010.374055
GGACGTG30300.010.3627936
AGGACCT20700.010.3568945
TTAGGAC18450.010.3343223
Produced by FastQC (version 0.11.5)