FastQCFastQC Report
Thu 26 May 2016
SRR1512691_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512691_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2383628
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT82890.3477472155890097No Hit
CTGTAGGACGTGGAATATGGCAAGA56050.23514575260904805No Hit
GGTATCAACGCAGAGTACTTTTTTT55660.23350959126172371No Hit
TATCAACGCAGAGTACTTTTTTTTT55100.23116023137838623No Hit
GTCCTACAGTGGACATTTCTAAATT52320.2194973376718179No Hit
GTCCTAAAGTGTGTATTTCTCATTT51350.21542791073103687No Hit
CTTTAGGACGTGAAATATGGCGAGG50050.2099740395732891No Hit
GTACATGGGAAGCAGTGGTATCAAC33520.14062597015977327No Hit
GTCCTACAGTGTGCATTTCTCATTT28600.11998516547045092No Hit
ACGCAGAGTACTTTTTTTTTTTTTT27360.11478301144306075No Hit
CTGAAGGACCTGGAATATGGCGAGA26250.11012624453144534No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCACG400.005261109714.2577814
TAGGACC9100.012.4298594
TAAGACG701.0858248E-412.2209544
GGCGAGG12350.011.45600919
GGACCTG18200.011.1171986
AGGACCT19250.011.1101915
TAAGACT2350.010.51637654
GTCCTAA15450.010.4719751
GGTTTAC1002.3524726E-510.4688961
TGTCGAG1201.5339756E-610.28670118
AGGACGT31350.010.2179125
ACCTTTT8000.010.20735715
CACCTTT8100.010.19963614
GACGTGA16850.010.1468777
TTAGGAC18200.010.1319023
TCGAAAC750.002659082410.12844416
CGCACTG856.561412E-410.0645275
GTAATAC1701.546141E-910.0643163
TGTAGGA31850.010.05919552
TCCCGTG856.604607E-410.0573457
Produced by FastQC (version 0.11.5)