FastQCFastQC Report
Thu 26 May 2016
SRR1512690_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512690_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2478366
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT95570.3856169750553389No Hit
CTGTAGGACGTGGAATATGGCAAGA87850.3544674192593023No Hit
GTCCTAAAGTGTGTATTTCTCATTT85960.3468414269724488No Hit
CTTTAGGACGTGAAATATGGCGAGG67150.27094464659376377No Hit
GTATCAACGCAGAGTACTTTTTTTT64610.2606959585468813No Hit
GTCCTACAGTGTGCATTTCTCATTT48120.19416018457322284No Hit
GGTATCAACGCAGAGTACTTTTTTT40700.16422110374335347No Hit
CTGAAGGACCTGGAATATGGCGAGA38310.1545776531795546No Hit
TATCAACGCAGAGTACTTTTTTTTT36660.14792004086563484No Hit
GTCCTTCAGTGTGCATTTCTCATTT33620.13565389454180699No Hit
CTGTAGGACCTGGAATATGGCGAGA32600.1315382796568384No Hit
ATTTAGAAATGTCCACTGTAGGACG31230.1260104439780081No Hit
GAATATGGCAAGAAAACTGAAAATC29190.11777921420807096No Hit
TTTCTAAATTTTCCACCTTTTTCAG26150.10551306788424308No Hit
GGAATATGGCGAGAAAACTGAAAAT24830.1001869780331073No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGG5300.013.5174771
TAGGACC13900.013.2409834
GGCGAGG21900.012.07429619
CGTTAAA550.003162493512.042729
CACCTTT12600.011.90038114
CCACCTT12250.011.53940613
GCGTAGG600.00461228611.4497351
ACCTTTT12750.011.38821215
TGTAGGA51900.011.2234272
ACCGTGC855.560345E-511.1292248
TCCAACG2250.010.94931518
TTCCACC13100.010.8591211
ACGGCGA700.001504615310.84613617
TAGGACT5600.010.8423954
GTAGGAC50350.010.8152583
AGGACGT54000.010.7520425
GGACGTG53150.010.6919486
GACGTGG27900.010.6772337
ATGTAGG5150.010.67159751
TTAGGAC29550.010.658723
Produced by FastQC (version 0.11.5)