FastQCFastQC Report
Thu 26 May 2016
SRR1512690_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512690_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2478366
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA107060.4319781662595436No Hit
GTCCTACAGTGGACATTTCTAAATT97360.39283947568680333No Hit
GTCCTAAAGTGTGTATTTCTCATTT87100.3514412318438842No Hit
GTATCAACGCAGAGTACTTTTTTTT82840.3342524873243096No Hit
CTTTAGGACGTGAAATATGGCGAGG80940.3265861458719172No Hit
GGTATCAACGCAGAGTACTTTTTTT56340.22732719864620476No Hit
TATCAACGCAGAGTACTTTTTTTTT55950.2257535811901874No Hit
GTCCTACAGTGTGCATTTCTCATTT48680.19641973784340166No Hit
CTGAAGGACCTGGAATATGGCGAGA48420.1953706595393901No Hit
CTGTAGGACCTGGAATATGGCGAGA40910.16506843621967054No Hit
ATTTAGAAATGTCCACTGTAGGACG35770.1443289651326721No Hit
TTTCTAAATTTTCCACCTTTTTCAG33570.13545214871411243No Hit
GAATATGGCAAGAAAACTGAAAATC33160.13379783292701725No Hit
GTCCTTCAGTGTGCATTTCTCATTT32470.1310137405048326No Hit
ACGCAGAGTACTTTTTTTTTTTTTT29120.11749677004929862No Hit
GGAATATGGCGAGAAAACTGAAAAT28790.11616524758651466No Hit
ATTCCAGGTCCTTCAGTGTGCATTT27480.11087950690091777No Hit
GTACATGGGAAGCAGTGGTATCAAC27380.1104760152455287No Hit
TTCCAGGTCCTTCAGTGTGCATTTC25840.10426224375253694No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACCG400.005256688714.259625
TAGGACC15250.013.1531344
TAGAAAT12500.012.0921564
AATGTCC12150.011.960418
CACCTTT11900.011.72994814
TTGGCGA658.0598425E-411.68650917
ACCTTTT12150.011.64442915
ATTTAGA12650.011.3615751
CCTTTTT13050.011.20516916
ATAGGAC4450.011.1083933
CGGTTTC1201.2826058E-711.07940213
CCACCTT12350.011.07299413
AAATGTC12400.011.0299037
GAAATGT13050.010.9910996
CCAACGA1658.185452E-1110.9339719
AGGACCT32250.010.8771055
ATGTCCA13550.010.8648399
CTTAGAC1752.0008883E-1110.8642523
CGAATGG1053.4889945E-610.85175919
CTTTTTC14850.010.80608717
Produced by FastQC (version 0.11.5)