FastQCFastQC Report
Thu 26 May 2016
SRR1512689_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512689_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2027131
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT74370.36687318185159223No Hit
GTCCTAAAGTGTGTATTTCTCATTT66200.3265699158071185No Hit
GTCCTACAGTGGACATTTCTAAATT65730.32425136806649396No Hit
CTGTAGGACGTGGAATATGGCAAGA60020.2960834795580552No Hit
CTTTAGGACGTGAAATATGGCGAGG51250.25282036533406077No Hit
GGTATCAACGCAGAGTACTTTTTTT49040.24191825787282617No Hit
TATCAACGCAGAGTACTTTTTTTTT45180.2228765679179096No Hit
GTACATGGGAAGCAGTGGTATCAAC40860.20156566102536047No Hit
GTCCTACAGTGTGCATTTCTCATTT36870.18188267063154773No Hit
CTGAAGGACCTGGAATATGGCGAGA28960.14286200546486635No Hit
CCCATGTACTCTGCGTTGATACCAC26900.13269986004851192No Hit
GTCCTTCAGTGTGCATTTCTCATTT26270.12959201946001517No Hit
ATTTAGAAATGTCCACTGTAGGACG25880.1276681181433267No Hit
CTGTAGGACCTGGAATATGGCGAGA25470.1256455552206542No Hit
GTACTTTTTTTTTTTTTTTTTTTTT24340.12007117448255687No Hit
GAATATGGCAAGAAAACTGAAAATC24210.1194298740436607No Hit
TTTCTAAATTTTCCACCTTTTTCAG22580.11138895315596278No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22520.11109296833801072No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTAG851.8170431E-713.8548761
TAGGACC9800.012.9727984
GTGTAGG3550.012.4401171
GTTCTAG1455.4569682E-1112.1827351
GGCGAGG16000.011.61017219
GTACTAG1103.2633034E-711.598211
TGCACCG752.0997753E-411.3850825
AAATGTC8400.011.1820557
GTTTAGG3350.011.1321511
AACCCCG600.00593423511.068835
GAAATGT8850.011.0420296
AATGTCC8400.011.0359718
CACCTTT9650.010.91587114
GACGTAA700.00150780510.84320457
CCCCGGT1408.6674845E-910.83164819
ACCTTTT10200.010.79246215
TAAGACG803.8176274E-410.6735154
GTCCTAA19700.010.6607511
TCCAACG2050.010.62902918
GTATTAT2150.010.4985591
Produced by FastQC (version 0.11.5)