FastQCFastQC Report
Thu 26 May 2016
SRR1512689_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512689_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2027131
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT88520.43667626808528903No Hit
CTGTAGGACGTGGAATATGGCAAGA74000.36504794214088776No Hit
GTCCTAAAGTGTGTATTTCTCATTT71510.3527645721958768No Hit
GTCCTACAGTGGACATTTCTAAATT68420.33752135407134515No Hit
CTTTAGGACGTGAAATATGGCGAGG64520.31828234090446056No Hit
GGTATCAACGCAGAGTACTTTTTTT62670.30915614235093836No Hit
TATCAACGCAGAGTACTTTTTTTTT60250.2972180880268715No Hit
GTACATGGGAAGCAGTGGTATCAAC45940.2266257089453025No Hit
GTCCTACAGTGTGCATTTCTCATTT39340.19406737897057466No Hit
CTGAAGGACCTGGAATATGGCGAGA35720.17620962828746636No Hit
CCCATGTACTCTGCGTTGATACCAC32280.1592398320582143No Hit
CTGTAGGACCTGGAATATGGCGAGA32210.1588945164372702No Hit
ACGCAGAGTACTTTTTTTTTTTTTT29880.14740043934013144No Hit
ATTTAGAAATGTCCACTGTAGGACG29730.14666047729525125No Hit
GAATATGGCAAGAAAACTGAAAATC27530.13580770063700867No Hit
GTACTTTTTTTTTTTTTTTTTTTTT27350.13491974618315244No Hit
TTTCTAAATTTTCCACCTTTTTCAG26770.13205855960961577No Hit
GTCCTTCAGTGTGCATTTCTCATTT25430.12544823200868616No Hit
GGAATATGGCGAGAAAACTGAAAAT22760.11227690760981901No Hit
GAGTACTTTTTTTTTTTTTTTTTTT21610.10660386526573763No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC11800.013.856714
CCAACGA1001.4530087E-712.34361919
GGCGAGG18400.012.17848719
CCACCTT9300.011.84596913
TCCAACG1900.011.49405818
GAAATGT10200.011.2687886
ACCTTTT9950.011.2605115
AGGACCT24200.011.2353295
TAGGACA9600.011.0907384
TAGAAAT10550.011.0832294
GTCCTAT3200.011.0028541
AAATGTC9700.010.9671927
GTCCTAG3150.010.8754081
CACCTTT10350.010.82612314
AATGTCC9750.010.8135318
GGACCTG23950.010.788366
TCCTAGA3000.010.7749242
GCCGGTT1502.1391315E-910.7648211
GTCCTAA19750.010.6964451
TGGCGAG42150.010.65522718
Produced by FastQC (version 0.11.5)