FastQCFastQC Report
Thu 26 May 2016
SRR1512688_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512688_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2181643
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT60070.2753429410769773No Hit
GTCCTACAGTGGACATTTCTAAATT55530.25453293687372314No Hit
GTCCTAAAGTGTGTATTTCTCATTT50890.23326456253383343No Hit
CTGTAGGACGTGGAATATGGCAAGA48070.2203385246807108No Hit
CTTTAGGACGTGAAATATGGCGAGG43220.19810757305388643No Hit
GGTATCAACGCAGAGTACTTTTTTT38230.17523490323577232No Hit
TATCAACGCAGAGTACTTTTTTTTT35120.16097959198640657No Hit
GTACATGGGAAGCAGTGGTATCAAC27030.12389744793259026No Hit
GTCCTACAGTGTGCATTTCTCATTT26900.12330156675496404No Hit
CTGAAGGACCTGGAATATGGCGAGA23400.10725861197271964No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC9450.012.6504054
TAGTACG600.00593338711.0691054
GTATAGG1603.092282E-1011.0400591
GTTCTAG1707.6397555E-1110.9679011
GCGCGCC1053.5458415E-610.83695811
TTAGGAC18200.010.7389423
AGGACCT19600.010.6979825
GGACCGT803.8170075E-410.67377956
GTCCTAA16100.010.6057711
GGACCGG909.642896E-510.5420056
GTCTACC750.002022449610.4676111
GACGTGA16700.010.3968147
GGCGAGG15200.010.35165619
GGACCTG20200.010.2393136
TAGAAAT9350.010.1473844
GTTCTAA3100.010.1299471
GTGTAGG3900.010.065011
CGTCGGT856.733166E-410.03619119
GTCTAGA2350.010.0221811
GTATTAT2850.09.985551
Produced by FastQC (version 0.11.5)