FastQCFastQC Report
Thu 26 May 2016
SRR1512688_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512688_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2181643
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT75620.346619497323806No Hit
GTCCTACAGTGGACATTTCTAAATT56710.25994170448602266No Hit
CTGTAGGACGTGGAATATGGCAAGA55840.2559538842972934No Hit
GTCCTAAAGTGTGTATTTCTCATTT53600.24568639323665695No Hit
CTTTAGGACGTGAAATATGGCGAGG51150.23445632488908588No Hit
GGTATCAACGCAGAGTACTTTTTTT50070.2295059274134219No Hit
TATCAACGCAGAGTACTTTTTTTTT48670.22308874550052413No Hit
GTACATGGGAAGCAGTGGTATCAAC30620.14035293583780664No Hit
CTGAAGGACCTGGAATATGGCGAGA28890.13242313247401155No Hit
GTCCTACAGTGTGCATTTCTCATTT28490.13058965192746935No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25400.11642601470543072No Hit
ATTTAGAAATGTCCACTGTAGGACG24130.11060471397015918No Hit
GTACTTTTTTTTTTTTTTTTTTTTT22960.10524178337152319No Hit
TTTCTAAATTTTCCACCTTTTTCAG22680.10395834698894366No Hit
CCCATGTACTCTGCGTTGATACCAC22330.10235405151071922No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGTAC655.425005E-513.1614383
TAGGACC9850.012.641814
TCCAACG1353.7471182E-1011.95724818
AGGACCT18550.010.8121665
TGTAGGA29100.010.7497092
CCTAATA1002.3771223E-510.4589122
GGACCTG18850.010.3808116
CTGTAGG29150.010.2511771
TAGAAAT7700.010.246174
CACCTTT8400.010.17464214
ATTTAGA8300.010.0899091
TTCCACC9250.010.06282411
GTATTAA2650.010.0552961
GTATAGC2650.010.0552961
GTAGGAC30200.010.0405613
ACCTTTT8550.09.99545215
CCAACGA951.6524602E-49.99522319
GGCGAGG13550.09.95096419
AAATGTC7850.09.92178257
TAGTACC1252.5518839E-69.8857024
Produced by FastQC (version 0.11.5)