FastQCFastQC Report
Thu 26 May 2016
SRR1512687_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512687_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3141132
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT122170.38893621789851557No Hit
CTGTAGGACGTGGAATATGGCAAGA106270.3383175237462163No Hit
GTCCTAAAGTGTGTATTTCTCATTT100510.3199801854872702No Hit
CTTTAGGACGTGAAATATGGCGAGG85360.2717491655874379No Hit
GTATCAACGCAGAGTACTTTTTTTT81290.25879205331071725No Hit
GTCCTACAGTGTGCATTTCTCATTT59310.188817279885086No Hit
GGTATCAACGCAGAGTACTTTTTTT48650.15488046984335585No Hit
CTGAAGGACCTGGAATATGGCGAGA47430.1509965197260096No Hit
TATCAACGCAGAGTACTTTTTTTTT46870.1492137229508343No Hit
CTGTAGGACCTGGAATATGGCGAGA41030.13062169943829166No Hit
GTCCTTCAGTGTGCATTTCTCATTT38610.12291747051699833No Hit
GTACATGGGAAGCAGTGGTATCAAC38510.12259911395000275No Hit
ATTTAGAAATGTCCACTGTAGGACG37120.118173957668764No Hit
GAATATGGCAAGAAAACTGAAAATC36470.11610463998329265No Hit
TTTCTAAATTTTCCACCTTTTTCAG34500.10983301561347948No Hit
GGAATATGGCGAGAAAACTGAAAAT31740.10104637436440113No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC18750.013.8133824
CGTTAGA450.00929788213.0824231
GTGTAGG5850.011.7406371
AATGTCC12250.011.6597158
TAGAAAT12950.011.5751334
AGGACCG1157.216113E-811.5496525
AAATGTC12200.011.4313147
TCCAACG4750.011.37118218
GATCAAC2950.011.3085361
TGTAGGA63600.011.0831552
AGGACCT40800.011.0684175
TTTAGAA14900.011.0151792
GGCGAGG28600.011.0029119
GGACCTG41050.011.0010086
ACTGTTC11900.010.889828
ATTTAGA13350.010.87752151
TAGGACT6300.010.8425294
GTCCTAT4800.010.8338821
CCAACGA3200.010.66321219
CTGTAGG57350.010.607371
Produced by FastQC (version 0.11.5)