FastQCFastQC Report
Thu 26 May 2016
SRR1512687_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512687_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3141132
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA128690.40969306606662825No Hit
GTCCTACAGTGGACATTTCTAAATT124440.3961629119693155No Hit
GTATCAACGCAGAGTACTTTTTTTT106640.3394954430441No Hit
GTCCTAAAGTGTGTATTTCTCATTT106070.33768081061222516No Hit
CTTTAGGACGTGAAATATGGCGAGG100570.3201711994274675No Hit
TATCAACGCAGAGTACTTTTTTTTT70630.2248552432689871No Hit
GGTATCAACGCAGAGTACTTTTTTT67990.21645062990030348No Hit
GTCCTACAGTGTGCATTTCTCATTT62340.19846348386505247No Hit
CTGAAGGACCTGGAATATGGCGAGA59970.19091843322725693No Hit
CTGTAGGACCTGGAATATGGCGAGA50430.16054721673587738No Hit
GTACATGGGAAGCAGTGGTATCAAC44390.14131848008934358No Hit
ATTTAGAAATGTCCACTGTAGGACG43940.1398858755378634No Hit
TTTCTAAATTTTCCACCTTTTTCAG43020.13695699512150397No Hit
GAATATGGCAAGAAAACTGAAAATC39920.1270879415446406No Hit
GTCCTTCAGTGTGCATTTCTCATTT39750.1265467353807481No Hit
GGAATATGGCGAGAAAACTGAAAAT35140.11187049764225127No Hit
ACGCAGAGTACTTTTTTTTTTTTTT33690.10725432742081517No Hit
ATTCCAGGTCCTTCAGTGTGCATTT33410.10636292903322751No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACGC400.00525762114.2594073
TAGGACC18450.013.1904844
TCCAACG3950.012.01869818
GGCGAGG27200.011.7986519
TAGAAAT14750.011.7299594
CCAACGA1900.011.493669519
TTTAGAA16050.011.4928282
AATCCCG4250.011.39372419
ACCTTTT14750.011.32935315
AAATGTC14600.011.3200097
CACCTTT15000.011.07846714
AATGTCC15200.010.9354838
AGGACCT37300.010.8830345
ATTTAGA16100.010.8764441
CCACCTT15300.010.862813
GAAATGT15850.010.847796
TGTAGGA67600.010.6897392
GCGTGCG803.7794176E-410.685539
GTCCTAA32150.010.6269461
CTGTAGG66950.010.5616851
Produced by FastQC (version 0.11.5)