FastQCFastQC Report
Thu 26 May 2016
SRR1512686_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512686_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3289360
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT79260.24095872753362355No Hit
GTCCTACAGTGGACATTTCTAAATT72050.21903957000753949No Hit
CTGTAGGACGTGGAATATGGCAAGA60880.18508159641997227No Hit
GTCCTAAAGTGTGTATTTCTCATTT60500.18392635649488046No Hit
GTACATGGGAAGCAGTGGTATCAAC56730.17246516039594328No Hit
CTTTAGGACGTGAAATATGGCGAGG47900.14562103266288884No Hit
GGTATCAACGCAGAGTACTTTTTTT47350.14394897487657174No Hit
TATCAACGCAGAGTACTTTTTTTTT45650.13878079626431888No Hit
GTCCTACAGTGTGCATTTCTCATTT40680.12367147408614441No Hit
CCCATGTACTCTGCGTTGATACCAC36900.11217987693654694No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC12750.012.79894354
CACCGTT604.1395667E-412.650127
GTCCTAC35600.011.0788261
TATACCG951.3812216E-510.985635
GTGTAGG5850.010.733511
AGGACCT24250.010.7199965
TCCTACA39850.010.6900682
TCCTATA4350.010.6873332
AAATGTC10250.010.552057
GTCCTAA16850.010.5389251
TGTAGGA40000.010.5076662
GTAGGAC38900.010.41455653
TAGAAAT10700.010.3742794
AATGTCC10550.010.3091268
TATGTCG1856.002665E-1110.26171816
GTATTAG2500.010.2035431
GTATTAT2700.010.1744731
GGACCTG24300.010.1513296
CCTACAG40750.010.1512933
GACGTGG19000.010.136747
Produced by FastQC (version 0.11.5)