FastQCFastQC Report
Thu 26 May 2016
SRR1512686_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512686_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3289360
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT97240.29561981662086245No Hit
GTCCTACAGTGGACATTTCTAAATT74430.22627502006469344No Hit
CTGTAGGACGTGGAATATGGCAAGA72440.2202252109832916No Hit
TATCAACGCAGAGTACTTTTTTTTT65520.1991876839263565No Hit
GTCCTAAAGTGTGTATTTCTCATTT64180.19511394313787483No Hit
GTACATGGGAAGCAGTGGTATCAAC64160.19505314103655422No Hit
GGTATCAACGCAGAGTACTTTTTTT64100.19487073473259234No Hit
CTTTAGGACGTGAAATATGGCGAGG59210.18000462095970035No Hit
CCCATGTACTCTGCGTTGATACCAC45340.13783836369384925No Hit
GTCCTACAGTGTGCATTTCTCATTT44940.13662232166743682No Hit
CTGAAGGACCTGGAATATGGCGAGA36440.11078142860617263No Hit
CTGTAGGACCTGGAATATGGCGAGA36230.1101430065423061No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC12950.011.6719244
GGCGAGG17200.011.2055819
TCCAACG2400.011.0767818
GTCCTAA18800.010.9342031
TGTAGGA36550.010.9255432
TAGGACA11200.010.8644314
TCTATAC4750.010.8070853
GTAGGAC36950.010.6510973
AGGACGT37250.010.64225
CTGTAGG35800.010.6333031
GGACGTG36000.010.5821226
GATATAC5950.010.55645751
TCCTATA3650.010.4195342
TTAGGAC23100.010.4115883
GACGTGA19950.010.2368667
TGGCGAG39400.010.19320918
GTCCTAC35900.010.1265181
TAGGACG39150.010.0770034
ATTTAGA10100.09.9879351
TTTAGGA24950.09.946062
Produced by FastQC (version 0.11.5)