##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512686_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3289360 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.732907313276748 33.0 31.0 34.0 30.0 34.0 2 31.857954130894765 34.0 31.0 34.0 30.0 34.0 3 31.960910633071478 34.0 31.0 34.0 30.0 34.0 4 35.40578866405623 37.0 35.0 37.0 33.0 37.0 5 35.18692815623708 37.0 35.0 37.0 33.0 37.0 6 35.05127258798064 37.0 35.0 37.0 32.0 37.0 7 35.03575193958703 37.0 35.0 37.0 32.0 37.0 8 35.02911387017535 37.0 35.0 37.0 32.0 37.0 9 36.63472529610623 39.0 37.0 39.0 32.0 39.0 10 36.442120047668844 39.0 37.0 39.0 32.0 39.0 11 36.553683391298 39.0 37.0 39.0 32.0 39.0 12 36.492199394411074 39.0 37.0 39.0 32.0 39.0 13 36.49174185859863 39.0 37.0 39.0 32.0 39.0 14 37.76258785903641 40.0 38.0 41.0 32.0 41.0 15 37.74736483692877 40.0 38.0 41.0 32.0 41.0 16 37.71108361504974 40.0 38.0 41.0 32.0 41.0 17 37.68519560035995 40.0 38.0 41.0 32.0 41.0 18 37.61594200695576 40.0 38.0 41.0 32.0 41.0 19 37.63088077923973 40.0 38.0 41.0 32.0 41.0 20 37.59201455602306 40.0 38.0 41.0 32.0 41.0 21 37.54925669431135 40.0 38.0 41.0 32.0 41.0 22 37.45360617262933 40.0 38.0 41.0 32.0 41.0 23 37.40000972833621 40.0 38.0 41.0 32.0 41.0 24 37.36093981808011 40.0 38.0 41.0 32.0 41.0 25 37.29495068949583 40.0 38.0 41.0 32.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5447514318345057 1101 2 0.5757608282783515 1101 3 0.5975990511522049 1101 4 0.48647852758066534 1101 5 0.49101846554583517 1101 6 0.6427880719309158 1101 7 0.5858937919212153 1101 8 0.6099398090546515 1101 9 0.6858127870768129 1101 10 0.8303024897136879 1101 11 0.8005006729025226 1101 12 0.7700983656104583 1101 13 0.7802681594641001 1101 14 0.9069255965604626 1101 15 0.9204593286387208 1101 16 0.8335610837429144 1101 17 0.8566991264742185 1101 18 0.810093412912515 1101 19 0.8621075368085087 1101 20 0.9095951839409153 1101 21 0.8519585906143234 1101 22 0.947649466232015 1101 23 0.989146923211436 1101 24 0.9162530388101828 1101 25 0.8998388883627939 1102 1 0.3945633437530205 1102 2 0.3125890730677163 1102 3 0.3311701597116503 1102 4 0.2362807066224022 1102 5 0.3196313536442901 1102 6 0.3778860909432282 1102 7 0.412477522680355 1102 8 0.29975711734433474 1102 9 0.3582347901523306 1102 10 0.5105596013022122 1102 11 0.4953375007001455 1102 12 0.5118026579803683 1102 13 0.5321954926453998 1102 14 0.6013549374449099 1102 15 0.6532544709913211 1102 16 0.6945315533575496 1102 17 0.7461375470956639 1102 18 0.7375707321224283 1102 19 0.6971073993304344 1102 20 0.6772022482017803 1102 21 0.6279110020796352 1102 22 0.5424017806920318 1102 23 0.5982501408928869 1102 24 0.6351025766222165 1102 25 0.6334886781450209 1103 1 0.4310026366429369 1103 2 0.5073359547773073 1103 3 0.4589839577102488 1103 4 0.4404707201377178 1103 5 0.4732906987038632 1103 6 0.5875742486437332 1103 7 0.5734573257226714 1103 8 0.528239344569613 1103 9 0.6642674478965844 1103 10 0.8153395509004255 1103 11 0.7024065684791552 1103 12 0.7032768705960208 1103 13 0.6737158023043861 1103 14 0.7305641985751592 1103 15 0.7956824018218853 1103 16 0.7398981154550839 1103 17 0.7008824487980334 1103 18 0.8243582499447015 1103 19 0.8009019417587169 1103 20 0.8488743554686735 1103 21 0.6649118568416839 1103 22 0.7504231081984898 1103 23 0.6905433163206425 1103 24 0.7452274850247846 1103 25 0.7806535994883532 1104 1 0.23090886220898454 1104 2 0.2870405869572643 1104 3 0.20190875026786514 1104 4 0.10852654146928131 1104 5 0.1601341369612399 1104 6 0.32442907317817316 1104 7 0.2937177987164148 1104 8 0.1982096190265139 1104 9 0.24892420143088145 1104 10 0.4310684587962683 1104 11 0.3292041499126839 1104 12 0.29734656846570573 1104 13 0.24483137171781522 1104 14 0.2672539004451977 1104 15 0.27116986869128823 1104 16 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-1.5862443853195671 2314 20 -1.6290402560711215 2314 21 -1.482001023674755 2314 22 -1.5258663201343055 2314 23 -1.339015767410423 2314 24 -1.5307339155318758 2314 25 -1.4766522471063155 2315 1 -0.6891948345068606 2315 2 -0.602015530758294 2315 3 -0.636172857382288 2315 4 -0.5697400910957526 2315 5 -0.6994141789962356 2315 6 -0.5910818544248073 2315 7 -0.7362955470552635 2315 8 -0.8374013704083225 2315 9 -0.9615984925556162 2315 10 -0.8201999642598068 2315 11 -0.8664465871338294 2315 12 -1.0874840354555104 2315 13 -1.0946676465140044 2315 14 -1.2480526503691252 2315 15 -1.177771700471041 2315 16 -1.1215834789416093 2315 17 -1.2909393453760885 2315 18 -1.1389351947745112 2315 19 -0.9633614036562506 2315 20 -1.0982527876129993 2315 21 -1.071518734837234 2315 22 -1.0093119541065718 2315 23 -0.9841048379851927 2315 24 -1.1112503537710836 2315 25 -1.0773625744745559 2316 1 -1.3528859365694572 2316 2 -1.1851691837345193 2316 3 -1.177477378934178 2316 4 -1.000906751784612 2316 5 -1.165087145015221 2316 6 -4.731449118431911 2316 7 -2.426144220390654 2316 8 -1.721228565160942 2316 9 -1.7190676391950532 2316 10 -1.4529672305321881 2316 11 -1.5734701352970362 2316 12 -1.74733448954877 2316 13 -1.6473068492378715 2316 14 -1.9524741526114298 2316 15 -1.9860181825303798 2316 16 -1.968263875691946 2316 17 -1.9950326973601875 2316 18 -2.0258090585975026 2316 19 -2.2322146270482364 2316 20 -2.2163773789982883 2316 21 -2.340430792455763 2316 22 -2.3438898043731555 2316 23 -2.49921403368041 2316 24 -2.3958742960921526 2316 25 -2.5207999153940435 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 29896.0 3 1.0 4 620.0 5 5120.0 6 6457.0 7 5170.0 8 3990.0 9 3575.0 10 3444.0 11 3280.0 12 3133.0 13 3035.0 14 3023.0 15 3086.0 16 3134.0 17 3309.0 18 3730.0 19 4277.0 20 5218.0 21 6810.0 22 8884.0 23 11093.0 24 13537.0 25 16128.0 26 18322.0 27 21007.0 28 24911.0 29 30015.0 30 38735.0 31 50973.0 32 65012.0 33 86347.0 34 115970.0 35 158044.0 36 236066.0 37 424281.0 38 1869727.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.342627408865134 18.620041404664985 12.820861033119165 25.216470153350723 2 15.402536439771186 19.91010048397842 40.487560292059406 24.19980278419099 3 19.22854293844395 24.348444682248218 29.4773755380986 26.94563684120923 4 12.819191499090369 16.548037727772428 37.24638580003697 33.386384973100235 5 13.314320339227038 37.919023635783184 35.40945449587516 13.357201529114624 6 33.02837034739077 36.5132271270157 17.28580813518495 13.172594390408584 7 28.85652989017639 31.14000343626414 21.57846161799386 18.425005055565606 8 25.795492384675384 37.03862171519279 19.722542790111405 17.44334311002042 9 26.831313137456437 14.556753009007256 19.5419132359828 39.0700206175535 10 15.514092925393113 28.574638968283583 33.96238865863668 21.948879447686625 11 35.16103437401067 21.645478346736265 24.268576438677446 18.92491084057562 12 24.157255874408424 24.018063125447426 31.420581063582226 20.404099936561924 13 29.850188495882524 21.330442191453923 25.30782998334279 23.51153932932076 14 21.19955877515106 21.804753139683296 26.924870767211935 30.0708173179537 15 23.443236472641264 30.89822634878362 22.732559907091566 22.925977271483553 16 22.45272211878845 27.36833272732358 27.767052688798387 22.41189246508958 17 22.25212711331866 28.35266409409613 27.402831651677236 21.992377140907976 18 22.236919709360652 26.55127230717782 29.7378469583194 21.473961025142128 19 24.16862476017218 26.13391322694316 27.27879370292937 22.418668309955283 20 24.319639273073957 26.54806804245692 27.726322279301918 21.40597040516721 21 23.249750711384586 26.031386044701705 26.991329620351678 23.72753362356203 22 23.93661830743605 27.41804513426854 26.71863452284211 21.926702035453303 23 22.540615803682176 27.31257752267918 27.592540798209985 22.554265875428655 24 23.74514305066732 26.210699409824223 28.05345965581744 21.99069788369102 25 23.145627112873022 26.65995816815429 27.683591944937618 22.510822774035073 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 209.0 1 209.0 2 482.5 3 756.0 4 756.0 5 756.0 6 2149.0 7 3542.0 8 3542.0 9 3542.0 10 4905.0 11 6268.0 12 6268.0 13 6268.0 14 9382.5 15 12497.0 16 12497.0 17 12497.0 18 22742.5 19 32988.0 20 32988.0 21 32988.0 22 52534.5 23 72081.0 24 72081.0 25 72081.0 26 106023.0 27 139965.0 28 139965.0 29 139965.0 30 183320.5 31 226676.0 32 226676.0 33 226676.0 34 257542.0 35 288408.0 36 288408.0 37 288408.0 38 313778.5 39 339149.0 40 339149.0 41 339149.0 42 363878.5 43 388608.0 44 388608.0 45 388608.0 46 415367.5 47 442127.0 48 442127.0 49 442127.0 50 431112.0 51 420097.0 52 420097.0 53 420097.0 54 377390.5 55 334684.0 56 334684.0 57 334684.0 58 291224.5 59 247765.0 60 247765.0 61 247765.0 62 206245.0 63 164725.0 64 164725.0 65 164725.0 66 129083.0 67 93441.0 68 93441.0 69 93441.0 70 69693.0 71 45945.0 72 45945.0 73 45945.0 74 32516.0 75 19087.0 76 19087.0 77 19087.0 78 13100.5 79 7114.0 80 7114.0 81 7114.0 82 4738.5 83 2363.0 84 2363.0 85 2363.0 86 1527.5 87 692.0 88 692.0 89 692.0 90 422.5 91 153.0 92 153.0 93 153.0 94 84.5 95 16.0 96 16.0 97 16.0 98 10.0 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15775105187635285 2 0.017541406230999344 3 0.0 4 0.003404917673954812 5 0.10752851618551938 6 0.043169491937641365 7 0.02751295084758129 8 0.028120971860787505 9 0.027360945594279738 10 0.028485784468711237 11 0.03493080720869713 12 0.03386677043558625 13 0.02109832915825571 14 0.010153950920543815 15 0.003040105066031082 16 0.002553688255466109 17 0.0013072451783933652 18 0.0018240630396186494 19 6.384220638665272E-4 20 6.080210132062164E-5 21 0.0 22 5.776199625459056E-4 23 0.0 24 3.040105066031082E-5 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3289360.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.57246642173539 #Duplication Level Percentage of deduplicated Percentage of total 1 78.01469562984717 48.03557227070671 2 13.724721219977909 16.901298729295377 3 4.01397321438047 7.414506928805603 4 1.6145603236910142 3.976498452653248 5 0.7912152219569939 2.435853634315646 6 0.4695678202651564 1.7347469307602292 7 0.3044434783405249 1.3121735093206806 8 0.19836963840552507 0.9771286319852788 9 0.1551899517020822 0.8599885281150472 >10 0.6546056281549566 6.945719998564988 >50 0.031216603857827607 1.31122607071305 >100 0.022057385502915 2.6835801596573794 >500 0.0027660321078957295 1.186279858683905 >1k 0.0022227043665918457 2.518419277611174 >5k 3.951474429496614E-4 1.7070070188117084 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 9724 0.29561981662086245 No Hit GTCCTACAGTGGACATTTCTAAATT 7443 0.22627502006469344 No Hit CTGTAGGACGTGGAATATGGCAAGA 7244 0.2202252109832916 No Hit TATCAACGCAGAGTACTTTTTTTTT 6552 0.1991876839263565 No Hit GTCCTAAAGTGTGTATTTCTCATTT 6418 0.19511394313787483 No Hit GTACATGGGAAGCAGTGGTATCAAC 6416 0.19505314103655422 No Hit GGTATCAACGCAGAGTACTTTTTTT 6410 0.19487073473259234 No Hit CTTTAGGACGTGAAATATGGCGAGG 5921 0.18000462095970035 No Hit CCCATGTACTCTGCGTTGATACCAC 4534 0.13783836369384925 No Hit GTCCTACAGTGTGCATTTCTCATTT 4494 0.13662232166743682 No Hit CTGAAGGACCTGGAATATGGCGAGA 3644 0.11078142860617263 No Hit CTGTAGGACCTGGAATATGGCGAGA 3623 0.1101430065423061 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 3.040105066031082E-5 6 0.0 0.0 0.0 0.0 3.040105066031082E-5 7 0.0 0.0 0.0 0.0 3.040105066031082E-5 8 0.0 0.0 0.0 0.0 6.080210132062164E-5 9 0.0 0.0 0.0 3.040105066031082E-5 6.080210132062164E-5 10 0.0 0.0 0.0 6.080210132062164E-5 6.080210132062164E-5 11 0.0 0.0 0.0 6.080210132062164E-5 6.080210132062164E-5 12 0.0 0.0 0.0 6.080210132062164E-5 9.120315198093246E-5 13 0.0 0.0 0.0 6.080210132062164E-5 1.2160420264124329E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 1295 0.0 11.671924 4 GGCGAGG 1720 0.0 11.20558 19 TCCAACG 240 0.0 11.07678 18 GTCCTAA 1880 0.0 10.934203 1 TGTAGGA 3655 0.0 10.925543 2 TAGGACA 1120 0.0 10.864431 4 TCTATAC 475 0.0 10.807085 3 GTAGGAC 3695 0.0 10.651097 3 AGGACGT 3725 0.0 10.6422 5 CTGTAGG 3580 0.0 10.633303 1 GGACGTG 3600 0.0 10.582122 6 GATATAC 595 0.0 10.5564575 1 TCCTATA 365 0.0 10.419534 2 TTAGGAC 2310 0.0 10.411588 3 GACGTGA 1995 0.0 10.236866 7 TGGCGAG 3940 0.0 10.193209 18 GTCCTAC 3590 0.0 10.126518 1 TAGGACG 3915 0.0 10.077003 4 ATTTAGA 1010 0.0 9.987935 1 TTTAGGA 2495 0.0 9.94606 2 >>END_MODULE